Bio-Protocol (May 2013)

Stem Microsome Preparation and Mannan Synthase Activity Assay

  • Yan Wang

DOI
https://doi.org/10.21769/bioprotoc.569
Journal volume & issue
Vol. 3, no. 10

Abstract

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Mannans are hemicellulosic polysaccharides and are present in cell walls of all land plants. Mannan polysaccharides are synthesized by two enzymes, mannan synthase (ManS) for backbone (mannan or glucomannan) synthesis and galactomannan galactosyl transferase for side-chain (galactosyl) addition. Here, a method for ManS activity assay using microsomes freshly isolated from Arabidopsis stems is described. This method can be applied to isolation of microsomes from any tissues of Arabidopsis or any other plants.