Replication of hepatitis C virus in cell culture

Biopolymers and Cell. 2009;25(1):44-49 DOI 10.7124/bc.0007C9


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Journal Title: Biopolymers and Cell

ISSN: 0233-7657 (Print); 1993-6842 (Online)

Publisher: Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine

LCC Subject Category: Science: Biology (General): Genetics

Country of publisher: Ukraine

Language of fulltext: English

Full-text formats available: PDF



Fedorchenko D. B.

Popova L. A.

Borovykov V. M.

Zavelevych M. P.

Diadiun S. T.

Alekseenko I. P.

Porva Yu. I.

Rybalko S. L.


Double blind peer review

Editorial Board

Instructions for authors

Time From Submission to Publication: 8 weeks


Abstract | Full Text

The technique for replicating hepatitis C virus (HCV) in cell culture has been modified and the susceptibility of the cells of various origin to HCV upon their infection with HCV-containing sera has been compared. The viral load on the fifth day post-infection has been assessed by reverse transcriptase polymerase chain reaction technique. The highest infection and replication efficacy have been found in cells of rat Gasser’s ganglion neurinoma. The peculiar features of the mitotic index and the anomalous forms of the mitosis have been studied in HCV-infected cells. The data presented may be used as a basis for the experimental model of HCV infection in vitro suitable for studying the effects of antiviral drugs on the infection caused by the cytopathogenic variant of HCV.