Secuenciamiento de las variantes del gen Kitlg a asociado a la pigmentación en el pez ornamental Platy (Xiphophorus maculatus, Günther, 1866)

Abstract

The production of ornamental fish is an important activity within Peruvian aquaculture generating positive socioeconomic impacts throughout the value chain. The ornamental Platyfish (Xiphophorus maculatus) is one of the most colorful ornamental fish widely used as an animal model system to understand the genetic basis of color pattern polymorphism. The coloration in X. maculatus is related to the synthesis of melanin pigments that affects body coloration. The amplification and molecular identification of the genetic polymorphism of the Kitlg a pigmentation gene sequence (Kit Ligando a) was achieved in two varieties of Platy fish (Tuxedo and Sunset Golden) that had 250 nucleotides. And whose sequences were compared with the Zebrafish (Danio rerio) sequence existing in the Genbank. Obtaining a consensus sequence of 176 nucleotides, the relationship of similarity being closer between the varieties of the Platy fish than with respect to D. rerio. This first report demonstrates the potential use of variability in the Kitlg a gene sequence from X. maculatus and provides enormous scope in the aquaculture field to improve the body pigmentation of other ornamental fish of commercial interest.

Keywords

• kitlg
• pigmentation
• genetic variability
• xiphophorus maculatus