This paper reports flow direction-dependent elastic instability in a symmetry-breaking microchannel. The microchannel consisted of a square chamber and a nozzle structure. A viscoelastic polyacrylamide solution was used for the instability demonstration. The instability was realized as the viscoelastic flow became asymmetric and unsteady in the microchannel when the flow exceeded a critical Weissenberg number. The critical Weissenberg number was found to be different for the forward-directed flow and the backward-directed flow in the microchannel.