Frontiers in Microbiology (Dec 2022)
Characteristics of intestinal microbiota in C57BL/6 mice with non-alcoholic fatty liver induced by high-fat diet
- Guangwen Yan,
- Shuaibing Li,
- Shuaibing Li,
- Yuhang Wen,
- Yuhang Wen,
- Yadan Luo,
- Yadan Luo,
- Jingrong Huang,
- Jingrong Huang,
- Baoting Chen,
- Baoting Chen,
- Shuya Lv,
- Shuya Lv,
- Lang Chen,
- Lang Chen,
- Lvqin He,
- Lvqin He,
- Manli He,
- Manli He,
- Qian Yang,
- Qian Yang,
- Zehui Yu,
- Zehui Yu,
- Wudian Xiao,
- Wudian Xiao,
- Yong Tang,
- Yong Tang,
- Weiyao Li,
- Jianhong Han,
- Jianhong Han,
- Fangfang Zhao,
- Shumin Yu,
- Fang Kong,
- Fang Kong,
- Benazir Abbasi,
- Hongmei Yin,
- Congwei Gu,
- Congwei Gu,
- Congwei Gu
Affiliations
- Guangwen Yan
- College of Animal Science, Xichang University, Xichang, China
- Shuaibing Li
- Laboratory Animal Centre, Southwest Medical University, Luzhou, China
- Shuaibing Li
- Model Animal and Human Disease Research of Luzhou Key Laboratory, Luzhou, China
- Yuhang Wen
- Laboratory Animal Centre, Southwest Medical University, Luzhou, China
- Yuhang Wen
- Model Animal and Human Disease Research of Luzhou Key Laboratory, Luzhou, China
- Yadan Luo
- Laboratory Animal Centre, Southwest Medical University, Luzhou, China
- Yadan Luo
- Model Animal and Human Disease Research of Luzhou Key Laboratory, Luzhou, China
- Jingrong Huang
- Laboratory Animal Centre, Southwest Medical University, Luzhou, China
- Jingrong Huang
- Model Animal and Human Disease Research of Luzhou Key Laboratory, Luzhou, China
- Baoting Chen
- Laboratory Animal Centre, Southwest Medical University, Luzhou, China
- Baoting Chen
- Model Animal and Human Disease Research of Luzhou Key Laboratory, Luzhou, China
- Shuya Lv
- Laboratory Animal Centre, Southwest Medical University, Luzhou, China
- Shuya Lv
- Model Animal and Human Disease Research of Luzhou Key Laboratory, Luzhou, China
- Lang Chen
- Laboratory Animal Centre, Southwest Medical University, Luzhou, China
- Lang Chen
- Model Animal and Human Disease Research of Luzhou Key Laboratory, Luzhou, China
- Lvqin He
- Laboratory Animal Centre, Southwest Medical University, Luzhou, China
- Lvqin He
- Model Animal and Human Disease Research of Luzhou Key Laboratory, Luzhou, China
- Manli He
- Laboratory Animal Centre, Southwest Medical University, Luzhou, China
- Manli He
- Model Animal and Human Disease Research of Luzhou Key Laboratory, Luzhou, China
- Qian Yang
- Laboratory Animal Centre, Southwest Medical University, Luzhou, China
- Qian Yang
- Model Animal and Human Disease Research of Luzhou Key Laboratory, Luzhou, China
- Zehui Yu
- Laboratory Animal Centre, Southwest Medical University, Luzhou, China
- Zehui Yu
- Model Animal and Human Disease Research of Luzhou Key Laboratory, Luzhou, China
- Wudian Xiao
- Laboratory Animal Centre, Southwest Medical University, Luzhou, China
- Wudian Xiao
- Model Animal and Human Disease Research of Luzhou Key Laboratory, Luzhou, China
- Yong Tang
- Model Animal and Human Disease Research of Luzhou Key Laboratory, Luzhou, China
- Yong Tang
- State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Taipa, Macao SAR, China
- Weiyao Li
- College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China
- Jianhong Han
- Laboratory Animal Centre, Southwest Medical University, Luzhou, China
- Jianhong Han
- Model Animal and Human Disease Research of Luzhou Key Laboratory, Luzhou, China
- Fangfang Zhao
- College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China
- Shumin Yu
- College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China
- Fang Kong
- Laboratory Animal Centre, Southwest Medical University, Luzhou, China
- Fang Kong
- Model Animal and Human Disease Research of Luzhou Key Laboratory, Luzhou, China
- Benazir Abbasi
- College of Veterinary Medicine, Nanjing Agricultural University, Jiangsu, China
- Hongmei Yin
- College of Animal Science, Xichang University, Xichang, China
- Congwei Gu
- Laboratory Animal Centre, Southwest Medical University, Luzhou, China
- Congwei Gu
- Model Animal and Human Disease Research of Luzhou Key Laboratory, Luzhou, China
- Congwei Gu
- College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China
- DOI
- https://doi.org/10.3389/fmicb.2022.1051200
- Journal volume & issue
-
Vol. 13
Abstract
IntroductionAs a representation of the gut microbiota, fecal and cecal samples are most often used in human and animal studies, including in non-alcoholic fatty liver disease (NAFLD) research. However, due to the regional structure and function of intestinal microbiota, whether it is representative to use cecal or fecal contents to study intestinal microbiota in the study of NAFLD remains to be shown.MethodsThe NAFLD mouse model was established by high-fat diet induction, and the contents of the jejunum, ileum, cecum, and colon (formed fecal balls) were collected for 16S rRNA gene analysis.ResultsCompared with normal mice, the diversity and the relative abundance of major bacteria and functional genes of the ileum, cecum and colon were significantly changed, but not in the jejunum. In NAFLD mice, the variation characteristics of microbiota in the cecum and colon (feces) were similar. However, the variation characteristics of intestinal microbiota in the ileum and large intestine segments (cecum and colon) were quite different.DiscussionTherefore, the study results of cecal and colonic (fecal) microbiota cannot completely represent the results of jejunal and ileal microbiota.
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