Journal of Asthma and Allergy (Sep 2022)

GARP Polymorphisms Associated with Susceptibility to House Dust Mite-Sensitized Persistent Allergic Rhinitis in a Chinese Population

  • Chen XY,
  • Zhu XJ,
  • Chen M,
  • Lu MP,
  • Wang ML,
  • Yin M,
  • Chen RX,
  • Wu ZF,
  • Bu DY,
  • Zhang ZD,
  • Cheng L

Journal volume & issue
Vol. Volume 15
pp. 1369 – 1381

Abstract

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Xin-Yuan Chen,1,* Xin-Jie Zhu,1,* Min Chen,1 Mei-Ping Lu,1 Mei-Lin Wang,2,3 Min Yin,1,4 Ruo-Xi Chen,1 Zhong-Fei Wu,1 Dong-Yun Bu,1 Zheng-Dong Zhang,2,3 Lei Cheng1,4 1Department of Otorhinolaryngology & Clinical Allergy Center, The First Affiliated Hospital, Nanjing Medical University, Nanjing, People’s Republic of China; 2Department of Environmental Genomics, Jiangsu Key Laboratory of Cancer Biomarkers, Prevention and Treatment, Collaborative Innovation Center for Cancer Personalized Medicine, School of Public Health, Nanjing Medical University, Nanjing, People’s Republic of China; 3Department of Genetic Toxicology, The Key Laboratory of Modern Toxicology of Ministry of Education, Center for Global Health, School of Public Health, Nanjing Medical University, Nanjing, People’s Republic of China; 4International Centre for Allergy Research, Nanjing Medical University, Nanjing, People’s Republic of China*These authors contributed equally to this workCorrespondence: Lei Cheng, Department of Otorhinolaryngology & Clinical Allergy Center, The First Affiliated Hospital, Nanjing Medical University, 300 Guangzhou Road, Nanjing, 210029, People’s Republic of China, Email [email protected] Zheng-Dong Zhang, Department of Environmental Genomics, Jiangsu Key Laboratory of Cancer Biomarkers, Prevention and Treatment, Collaborative Innovation Center for Cancer Personalized Medicine, School of Public Health, Nanjing Medical University, 101 Longmian Avenue, Nanjing, 211166, People’s Republic of China, Email [email protected]: Genetic variants in GARP (also known as LRRC32) have been reported to have significant associations with asthma and eczema in special populations, but little is known about allergic rhinitis. This study purposes to evaluate the association of single nucleotide polymorphisms (SNPs) in GARP with house dust mite (HDM)-sensitized persistent allergic rhinitis (PER) in a population of Han Chinese.Methods: In this hospital-based case–control study, 534 HDM-sensitized PER patients and 451 healthy controls were recruited from East China. In this population, six SNPs in GARP were identified. Serum total and specific IgE levels were measured with ImmunoCAP. Secondary structure and minimum free energy were predicted by RNAfold.Results: rs79525962 was associated with the risk of HDM-sensitized PER (P 0.05). rs79525962 increased the risk of HDM-sensitized PER in the subgroup aged ≥ 16 years (adjusted OR = 1.745, 95% CI = 1.103– 2.760), and this high risk was also found in the females (adjusted OR = 1.708, 95% CI = 1.021– 2.856). The G-C haplotype of rs1320646-rs3781699 rendered a lower risk of HDM-sensitized PER than the common haplotype G-A (adjusted OR = 0.819, 95% CI = 0.676– 0.993). The secondary structure of GARP altered in response to different genotypes of rs79525962 and rs3781699.Conclusion: SNP rs79525962 in the GARP gene marks a risk locus of HDM-sensitized PER in Chinese Hans.Keywords: allergic rhinitis, mites, GARP, LRRC32, single nucleotide polymorphism, genetic association studies

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