Mediators of Inflammation (Jan 2009)

Persistently Elevated Level of IL-8 in Chlamydia trachomatis Infected HeLa 229 Cells is Dependent on Intracellular Available Iron

  • Harsh Vardhan,
  • Raini Dutta,
  • Vikas Vats,
  • Rishein Gupta,
  • Rajneesh Jha,
  • Hem Chandra Jha,
  • Pragya Srivastava,
  • Apurb Rashmi Bhengraj,
  • Aruna Singh Mittal

DOI
https://doi.org/10.1155/2009/417658
Journal volume & issue
Vol. 2009

Abstract

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Chlamydia trachomatis is a leading cause of sexually transmitted infection worldwide and responsible for myriad of immunopathological changes associated with reproductive health. Delayed secretion of proinflammatory chemokine interleukin (IL)-8 is a hallmark of chlamydial infection and is dependent on chlamydial growth. We examined the effect of iron chelators on IL-8 production in HeLa 229 (cervix epitheloid cell, CCL2) cells infected with C. trachomatis. IL-8 production was induced by Iron chelator DFO and Mimosine, however, synergy with chlamydial infection was obtained with DFO only. Temporal expression of proinflammatory secreted cytokines IL-1beta, TNF-alpha, and IL-8 did not show synchrony in Chlamydia trachomatis infected cells. Secretion of IL-8 from Hela cells infected with C. trachomatis was not dependent on IL-1 beta and TNF- alpha induction. These results indicate towards involvement of iron in chlamydia induced IL-8 production.