Evaluation of Antioxidant and Antimicrobial Activity of Some Plants Collected from Malaysia

Abstract

Five plant species namely, Phyllanthus acidus, Piper aduncum, Pandanus amaryllifolius, Macaranga peltata and Acacia mangium were analysed for their effective in-vitro bioactivity. The chloroform and aqueous extracted of the selected plants were subjected to TLC bioautography for antioxidant activity later all the extracted were subjected for DPPH assay where the chloroform extracts were found to express maximum antioxidant property. Amongst all the plants, Macaranga peltata accounted to 95% DPPH scavenging activity. The antimicrobial studies of the plant extracts were performed via agar well diffusion method, MIC determination, Biofilm inhibition assay in microtitre plate against clinical isolates like Staphylococcus aureus (MRSA) and Pseudomonas aeruginosa. It was found that Piper aduncum (chloroform and aqueous extract) and Macaranga peltata (only aqueous) expressed antibacterial activity, of which only chloroform extract of Piper aduncum could show negative influence against the biofilm development of P.aeruginosa.

Keywords

• dpph scavenging
• antibacterial activity
• staphylococcus aureus
• pseudomonas aeruginosa