Association between Fat Mass and Obesity Associated (FTO) gene polymorphism (rs9939609) and lipid profile in type 2 diabetic obese Iraqi male

Abstract

Objective Investigation the risk of allele frequency of FTO gene polymorphism rs9939609 in obese male with type 2 diabetes mellitus (T2DM) and to examine the association of this polymorphism of FTO gene with BMI and lipid profile in obese Iraqi male. Methods The study included 120 patients (males) with T2DM and 60 healthy controls to explore the relation of these SNPs with T2DM in obese male in Iraqi population. The patient’s group was enrolled from Al-Husain medical city in Karbala province based on WHO guidelines of T2DM. BMI, fasting blood sugar (FBS), lipid profile and HbA1c were measured, DNA was isolated from whole blood and the FTO gene variant (rs9939609) was genotyped by using ARMS–PCR technique with using specific primers. Multinomial logistic regression was applied to compare the proportions of genotypes or alleles. The odds ratio, t-test, and P-value at 95% confidence interval (CI) were measured before and after adjustment of BMI and age. Also in the present study, the Hardy Weinberg equilibrium was tested. Results The results of our study showed there are no significant differences between the SNP rs9939609 in the FTO gene and T2DM in Iraqi obese male, and the genotyping results of rs9939609 was consistent with Hardy–Weinberg equilibrium in obese T2DM, non-obese T2DM, and control individuals (P = 0.05), (P = 0.46) and (P = 0.002), respectively. The results show that the studied SNP of FTO gene have significant association with BMI and HDL-C level, but not have association with other parameters in obese diabetic patients group, while it is exhibited failure of FTO gene polymorphism to affect any of the measured clinical characteristics in non-obese diabetic patients. Conclusions The FTO gene polymorphism rs9939609 have significant association with HDL-C level in obese diabetic Iraqi male but does not affect other tested biochemical parameters.