Cucumeropsis mannii seed oil (CMSO) attenuates alterations in testicular biochemistry and histology against Bisphenol a-induced toxicity in male Wister albino rats
P.C. Agu,
P.M. Aja,
Ezebuilo Ekpono Ugbala,
H.A. Ogwoni,
E.M. Ezeh,
P.C. Oscar-Amobi,
Agbor Asuk Atamgba,
O.G. Ani,
J.N. Awoke,
F.E. Nwite,
O.U. Ukachi,
O.U. Orji,
P.C. Nweke,
Ejike Ekpono Ugbala,
G.O. Ewa,
I.O. Igwenyi,
C.O. Egwu,
E.U. Alum,
D.C. Chukwu,
A.C. Famurewa
Affiliations
P.C. Agu
Department of Biochemistry, Faculty of Science, Ebonyi State University, Abakaliki, Nigeria; Corresponding author.
P.M. Aja
Department of Biochemistry, Faculty of Science, Ebonyi State University, Abakaliki, Nigeria
Ezebuilo Ekpono Ugbala
Department of Science Laboratory Technology, Biochemistry Option, Federal Polytechnic Oko, Anambra State, Nigeria
H.A. Ogwoni
Department of Biochemistry, Faculty of Science, Ebonyi State University, Abakaliki, Nigeria
E.M. Ezeh
Department of Chemical Engineering, Caritas University, Amorji-Nike, Enugu State, Nigeria
P.C. Oscar-Amobi
Department of Biochemistry, Faculty of Science, Ebonyi State University, Abakaliki, Nigeria
Agbor Asuk Atamgba
Department of Medical Biochemistry, Faculty of Basic Medical Sciences, Cross River University of Technology (CRUTECH), Nigeria
O.G. Ani
Department of Clinical Sciences and Nutrition, University of Chester, Parkgate Road Tower Building United Kingdom; Department of Biochemistry, Faculty of Science, Ebonyi State University, Abakaliki, Nigeria
J.N. Awoke
Department of Biochemistry, Faculty of Science, Ebonyi State University, Abakaliki, Nigeria
F.E. Nwite
Department of Biochemistry, Faculty of Science, Ebonyi State University, Abakaliki, Nigeria
O.U. Ukachi
Department of Biochemistry, Faculty of Science, Ebonyi State University, Abakaliki, Nigeria
O.U. Orji
Department of Biochemistry, Faculty of Science, Ebonyi State University, Abakaliki, Nigeria
P.C. Nweke
Department of Biochemistry, Faculty of Science, Ebonyi State University, Abakaliki, Nigeria
Ejike Ekpono Ugbala
Department of Biochemistry, Faculty of Science, Ebonyi State University, Abakaliki, Nigeria
G.O. Ewa
Department of Biochemistry, Faculty of Science, Ebonyi State University, Abakaliki, Nigeria
I.O. Igwenyi
Department of Biochemistry, Faculty of Science, Ebonyi State University, Abakaliki, Nigeria
C.O. Egwu
Department of Medical Biochemistry, Faculty of Basic Medical Sciences, College of Medicine, Alex-Ekwueme Federal University, Ndufu-Alike, Ikwo, Ebonyi State, Nigeria
E.U. Alum
Department of Biochemistry, Faculty of Science, Ebonyi State University, Abakaliki, Nigeria
D.C. Chukwu
Department of Biochemistry, Faculty of Science, Ebonyi State University, Abakaliki, Nigeria
A.C. Famurewa
Department of Medical Biochemistry, Faculty of Basic Medical Sciences, College of Medicine, Alex-Ekwueme Federal University, Ndufu-Alike, Ikwo, Ebonyi State, Nigeria
Background: Male reproductive health has deteriorated in recent years as a result of industrialization, which has led to the use of desirable chemicals, like Bisphenol A (BPA), of underlying toxicity. Cucumeropsis mannii seed is a common soup thickener that produces vegetable oil as well as essential nutrients making it a source of nutraceuticals enlisted with a wide range of therapeutic effects. Methods: A total of 48 adult male Wistar rats (120 ± 200g) were used in this study. They were completely randomized and divided into six groups: A (1ml olive oil) irrespective of the weight, B [BPA 100 mg/kg body weight (bw)], C (CMSO 7.5 ml/kg bw), D (CMSO 7.5 ml/kg bw + BPA 100 mg/kg bw), E (CMSO 5.0 ml/kg bw + BPA 100 mg/kg bw), and E (CMSO 2.5 ml/kg bw + BPA 100 mg/kg bw). At the end of the administration via oral routes, rats were sacrificed and testes were collected for biochemistry and histological analysis. Results: BPA significantly (P < 0.05) decreased total testicular protein, epididymal sperm parameters (count, volume, and motility), Mitochondrial Membrane Potential (MMP), body weight, testicular volume; and significantly (P < 0.05) increased testicular enzymes (alkaline phosphatase and lactate dehydrogenase), testicular index; plus histological damages. Interestingly, co-administration of BPA and CMSO significantly (P < 0.05) reversed the biochemical and histological changes. Conclusions: CMSO prevented the biochemistry and histological alterations hence reducing the testicular toxicity. Therefore, CMSO has the potential to be a promising novel nutraceutical for the treatment and management of BPA-induced testicular toxicity.
