Synergistic Biocontrol and Growth Promotion in Strawberries by Co-Cultured Trichoderma harzianum TW21990 and Burkholderia vietnamiensis B418

Wenzhe Li; Yiting Fu; Yanqing Jiang; Jindong Hu; Yanli Wei; Hongmei Li; Jishun Li; Hetong Yang; Yuanzheng Wu

doi:10.3390/jof10080551

Journal of Fungi (Aug 2024)

Synergistic Biocontrol and Growth Promotion in Strawberries by Co-Cultured Trichoderma harzianum TW21990 and Burkholderia vietnamiensis B418

Wenzhe Li,
Yiting Fu,
Yanqing Jiang,
Jindong Hu,
Yanli Wei,
Hongmei Li,
Jishun Li,
Hetong Yang,
Yuanzheng Wu

Affiliations

Wenzhe Li: School of Bioengineering, Qilu University of Technology, Shandong Academy of Sciences, Jinan 250353, China
Yiting Fu: School of Bioengineering, Qilu University of Technology, Shandong Academy of Sciences, Jinan 250353, China
Yanqing Jiang: School of Bioengineering, Qilu University of Technology, Shandong Academy of Sciences, Jinan 250353, China
Jindong Hu: Shandong Provincial Key Laboratory of Applied Microbiology, Ecology Institute, Qilu University of Technology, Shandong Academy of Sciences, Jinan 250103, China
Yanli Wei: Shandong Provincial Key Laboratory of Applied Microbiology, Ecology Institute, Qilu University of Technology, Shandong Academy of Sciences, Jinan 250103, China
Hongmei Li: Shandong Provincial Key Laboratory of Applied Microbiology, Ecology Institute, Qilu University of Technology, Shandong Academy of Sciences, Jinan 250103, China
Jishun Li: Shandong Provincial Key Laboratory of Applied Microbiology, Ecology Institute, Qilu University of Technology, Shandong Academy of Sciences, Jinan 250103, China
Hetong Yang: Shandong Provincial Key Laboratory of Applied Microbiology, Ecology Institute, Qilu University of Technology, Shandong Academy of Sciences, Jinan 250103, China
Yuanzheng Wu: Shandong Provincial Key Laboratory of Applied Microbiology, Ecology Institute, Qilu University of Technology, Shandong Academy of Sciences, Jinan 250103, China

DOI: https://doi.org/10.3390/jof10080551
Journal volume & issue: Vol. 10, no. 8
p. 551

Abstract

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This study aimed to investigate the efficiency of the secondary metabolites (SMs) produced by a co-culture of Trichoderma harzianum TW21990 and Burkholderia vietnamiensis B418 in the control of Colletotrichum siamense CM9. A fermentation filtrate of B418 + TW21990 co-culture (BT21) produced a notable increase in the inhibition rate of CM9 compared to those of TW21990 and B418 monocultures, which reached 91.40% and 80.46% on PDA plates and strawberry leaves, respectively. The BT21 fermentation broth exhibited high control efficiency on strawberry root rot of 68.95% in a pot experiment, which was higher than that in the monocultures and fluazinam treatment. In addition, BT21 treatment promoted strawberry root development, improved antioxidative enzyme activities in the leaves and roots, and enhanced the total chlorophyll content of the strawberry leaves. UHPLC-MS/MS analysis of fermentation filtrates was performed to elucidate SM variations, revealing 478 and 795 metabolites in BT21 co-culture in positive and negative ion modes, respectively. The metabolomic profiles suggested abundant SMs with antagonistic capabilities and growth-promoting effects: 3-(propan-2-yl)-octahydropyrrolo [1,2-a]pyrazine-1,4-dione (cyclo(L-Pro-L-Val)), 3-[(4-hydroxyphenyl)methyl]-octahydropyrrolo[1,2-a]pyrazine-1,4-dione (cyclo(L-Pro-L-Tyr)), 3-indoleacetic acid (IAA), 2-hydroxycinnamic acid, 4-aminobutyric acid (GABA), bafilomycin B1, and DL-indole-3-lactic acid (ILA) were significantly enhanced in the co-culture. Overall, this study demonstrates that a co-culture strategy is efficient for inducing bioactive SMs in T. harzianum and B. vietnamiensis, which could be exploited as a novel approach for developing biocontrol consortia.

Published in Journal of Fungi

ISSN: 2309-608X (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Biology (General)
Website: http://www.mdpi.com/journal/jof

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