Offspring production from cryopreserved primordial germ cells in Drosophila

Abstract

Asaoka, Sakamaki, Fukumoto et al. present a more viable method of long-term storage of Drosophila fly strains by cryopreservation of primordial germ cells (PGCs) without any drop in viability. The authors show that PGCs from stage 5 embryos can be transplanted into embryos and properly developed into germline stem cells to produce offspring of both sexes after being revived from storage in liquid nitrogen.