Viruses
(Dec 2022)
Monoclonal Antibodies Specific for SARS-CoV-2 Spike Protein Suitable for Multiple Applications for Current Variants of Concern
Mahali S. Morgan,
Kexin Yan,
Thuy T. Le,
Ryan A. Johnston,
Alberto A. Amarilla,
David A. Muller,
Christopher L. D. McMillan,
Naphak Modhiran,
Daniel Watterson,
James R. Potter,
Julian D.J. Sng,
Mary Lor,
Devina Paramitha,
Ariel Isaacs,
Alexander A. Khromykh,
Roy A. Hall,
Andreas Suhrbier,
Daniel J. Rawle,
Jody Hobson-Peters
Affiliations
Mahali S. Morgan
School of Chemistry and Molecular Biosciences, University of Queensland, St Lucia, QLD 4072, Australia
Kexin Yan
Inflammation Biology, QIMR Berghofer Medical Research Institute, Herston, QLD 4006, Australia
Thuy T. Le
Inflammation Biology, QIMR Berghofer Medical Research Institute, Herston, QLD 4006, Australia
Ryan A. Johnston
School of Chemistry and Molecular Biosciences, University of Queensland, St Lucia, QLD 4072, Australia
Alberto A. Amarilla
School of Chemistry and Molecular Biosciences, University of Queensland, St Lucia, QLD 4072, Australia
David A. Muller
School of Chemistry and Molecular Biosciences, University of Queensland, St Lucia, QLD 4072, Australia
Christopher L. D. McMillan
School of Chemistry and Molecular Biosciences, University of Queensland, St Lucia, QLD 4072, Australia
Naphak Modhiran
School of Chemistry and Molecular Biosciences, University of Queensland, St Lucia, QLD 4072, Australia
Daniel Watterson
School of Chemistry and Molecular Biosciences, University of Queensland, St Lucia, QLD 4072, Australia
James R. Potter
School of Chemistry and Molecular Biosciences, University of Queensland, St Lucia, QLD 4072, Australia
Julian D.J. Sng
School of Chemistry and Molecular Biosciences, University of Queensland, St Lucia, QLD 4072, Australia
Mary Lor
Inflammation Biology, QIMR Berghofer Medical Research Institute, Herston, QLD 4006, Australia
Devina Paramitha
School of Chemistry and Molecular Biosciences, University of Queensland, St Lucia, QLD 4072, Australia
Ariel Isaacs
School of Chemistry and Molecular Biosciences, University of Queensland, St Lucia, QLD 4072, Australia
Alexander A. Khromykh
School of Chemistry and Molecular Biosciences, University of Queensland, St Lucia, QLD 4072, Australia
Roy A. Hall
School of Chemistry and Molecular Biosciences, University of Queensland, St Lucia, QLD 4072, Australia
Andreas Suhrbier
Inflammation Biology, QIMR Berghofer Medical Research Institute, Herston, QLD 4006, Australia
Daniel J. Rawle
Inflammation Biology, QIMR Berghofer Medical Research Institute, Herston, QLD 4006, Australia
Jody Hobson-Peters
School of Chemistry and Molecular Biosciences, University of Queensland, St Lucia, QLD 4072, Australia
DOI
https://doi.org/10.3390/v15010139
Journal volume & issue
Vol. 15,
no. 1
Abstract
Read online
The global coronavirus disease 2019 (COVID-19) pandemic caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has spawned an ongoing demand for new research reagents and interventions. Herein we describe a panel of monoclonal antibodies raised against SARS-CoV-2. One antibody showed excellent utility for immunohistochemistry, clearly staining infected cells in formalin-fixed and paraffin embedded lungs and brains of mice infected with the original and the omicron variants of SARS-CoV-2. We demonstrate the reactivity to multiple variants of concern using ELISAs and describe the use of the antibodies in indirect immunofluorescence assays, Western blots, and rapid antigen tests. Finally, we illustrate the ability of two antibodies to reduce significantly viral tissue titers in K18-hACE2 transgenic mice infected with the original and an omicron isolate of SARS-CoV-2.
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