In-vitro acaricidal activity of peganum harmala and glaucium flavum alkaloid against rhipicephalus sp. of dog

Abstract

Aim: The major problem of drug intended for tick control is resistance developed to application doses on the hosts. The acaricidal effects of alkaloids from Glaucium flavum and Peganum harmala were evaluated in vitro on adult female of Rhipicephalus sp. collected in naturally infected dogs. Materials and Methods: The acaricide activities of the both extracts (3.12, 6.2, 12.5, 25 and 50 mg/ml) were evaluated using the adult immersion test, reproductive index and inhibition oviposition. DMSO (1%) and SEB (1?l/ml) were used as a negative and positive control. Results: The percent mortality caused by the methanolic extracts of both plants varied from 41.66 to 75 %, when tested at concentrations ranging from 3.12 to 50 mg/ml, and significantly different compared to control (p Conclusion: The total alkaloids extracted from the G. flavum root and P. harmala seed have good acaricidal activities in vitro reducing potentially the egg laying of Rhipicephalus sp. females.

Keywords

• alkaloid
• acaricide
• in vitro
• rhipicephalus sp.
• dogs