EVALUATION OF UTERINE EXPRESSION OF SIRTUIN3 (SIRT3) mRNA IN MALABARI AND ATTAPPADY BLACK GOATS OF KERALA*

Abstract

The sirtuins are a family of highly conserved NAD+-dependent deacetylases which are potent cellular regulators and have been implicated askey inhibitors of inflammation. Sirtuin3 (SIRT3),a member of the sirtuin family, prevents the activation of reactive oxygen species (ROS) thereby protecting the cell from oxidative stress. A study was conducted to comparetherelativeabundanceofSIRT3mRNA in uterine tissues of two native goat breeds of Kerala, Malabari and Attappady Black. The mRNA isolated from uterine tissues of Malabari and Attappady Black goats weresubjected to quantitative PCR (qPCR). The relative abundance of SIRT3 mRNA was significantly (p<0.05) higher in Attappady Black goats when compared with Malabari goats.SIRT3 has a protective role on the uterus by preventing the activation of reactive oxygen species (ROS) and thus preventing inflammatory process.The results of the present study suggest that SIRT3 gene plays an important role in the protection of uterine tissues in Attappady Black goats by its upregulation in the uterine tissues and can be considered as a potent candidate gene for reproductive traits in goats.

Keywords

• sirtuin3; malabari goats
• attappady black
• uterine expression