Nanowarming improves survival of vitrified ovarian tissue and follicular development in a sheep model
Sareh Karimi,
Seyed Nasrollah Tabatabaei,
Marefat Ghaffari Novin,
Mahsa Kazemi,
Zahra Shams Mofarahe,
Alireza Ebrahimzadeh-Bideskan
Affiliations
Sareh Karimi
Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran; Department of Anatomy and Cell Biology, School of Medicine, Mashhad University of Medical Sciences, Iran
Seyed Nasrollah Tabatabaei
Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran; Department of Pediatrics, Physiology and Pharmacology, University of Montreal, Montreal, Qc, Canada
Marefat Ghaffari Novin
Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Mahsa Kazemi
Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Zahra Shams Mofarahe
Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran; Corresponding author. Department of Biology and Anatomical Sciences, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Velenjak, Tehran, Iran.
Alireza Ebrahimzadeh-Bideskan
Department of Anatomy and Cell Biology, School of Medicine, Mashhad University of Medical Sciences, Iran; Applied Biomedical Research Center, Mashhad University of Medical Sciences, Mashhad, Iran; Corresponding author. Department of Anatomy and Cell Biology, School of Medicine, Mashhad University of Medical Sciences, Azadi Sq., Vakilabad Blvd., P.O. Box 91779-48564, Mashhad, Iran.
Tissue cryopreservation has allowed long term banking of biomaterials in medicine. Ovarian tissue cryopreservation in particular helps patients by extending their fertility window. However, protection against tissue injury during the thawing process has proven to be challenging. This is mainly due to the heterogenous and slow distribution of the thermal energy across the vitrified tissue during a conventional warming process. Nanowarming is a technique that utilizes hyperthermia of magnetic nanoparticles to accelerate this process. Herein, hyperthermia of synthesized PEGylated silica-coated iron oxide nanoparticles was used to deter the injury of cryopreserved ovarian tissue in a sheep model. When compared to the conventional technique, our findings suggest that follicular development and gene expression in tissues warmed by the proposed technique have been improved. In addition, Nanowarming prevented cellular apoptosis and oxidative stress. We therefore conclude that Nanowarming is a potential complementary candidate to increase efficiency in the ovarian cryopreservation field.
