MicroRNA-154-5p suppresses cervical carcinoma growth and metastasis by silencing Cullin2 in vitro and in vivo
Yaqin Li,
Yimiao Wei,
Honglei Zhang,
Ying Bai,
Xiuting Wang,
Qi Li,
Yatao Liu,
Shuling Wang,
Jiapu Wang,
Songquan Wen,
Jiarong Li,
Weihong Zhao
Affiliations
Yaqin Li
Department of Obstetrics and Gynecology, The Second Hospital of Shanxi Medical University, Taiyuan, Shanxi Province, China
Yimiao Wei
Department of Obstetrics and Gynecology, The Second Hospital of Shanxi Medical University, Taiyuan, Shanxi Province, China
Honglei Zhang
Department of Pathology and Pathophysiology,Basic Medical College, Shanxi Medical University, Taiyuan, Shanxi Province, China
Ying Bai
Department of Obstetrics and Gynecology, The Second Hospital of Shanxi Medical University, Taiyuan, Shanxi Province, China
Xiuting Wang
Department of Biochemistry and Molecular Biology,Basic Medical College, Shanxi Medical University, Taiyuan, Shanxi Province, China
Qi Li
Department of Obstetrics and Gynecology, The Second Hospital of Shanxi Medical University, Taiyuan, Shanxi Province, China
Yatao Liu
Department of Obstetrics and Gynecology, The Second Hospital of Shanxi Medical University, Taiyuan, Shanxi Province, China
Shuling Wang
Department of Epidemiology,School of Public Health, Shanxi Medical University, Taiyuan, Shanxi Province, China
Jiapu Wang
Scientific Research Experiment Center, Central laboratory, The Affiliated Cardiovascular Hospital of Shanxi Medical University, Taiyuan, Shanxi Province, China
Songquan Wen
Department of Obstetrics and Gynecology, The Second Hospital of Shanxi Medical University, Taiyuan, Shanxi Province, China
Jiarong Li
Department of Epidemiology,School of Public Health, Shanxi Medical University, Taiyuan, Shanxi Province, China
Weihong Zhao
Department of Obstetrics and Gynecology, The Second Hospital of Shanxi Medical University, Taiyuan, Shanxi Province, China
Background MicroRNA-154-5p (miR-154-5p) plays a role in tumorigenesis in diverse human malignancies. Nevertheless, little is known about the mechanism by which miR-154-5p alters the growth and metastasis of cervical cancer. This research aimed to analyze the role of miR-154-5p in the pathology of cervical cancer in vitro and in vivo. Methods The level of miR-154-5p in human papillomavirus 16 positive cervical cancer cells was examined by real-time quantitative polymerase chain reaction. Bioinformatics predicted the downstream targets and potential functions of miR-154-5p. Furthermore, lentiviral technology was used to construct SiHa cell lines with stable up- and down-expression levels of miR-154-5p. Its differential expression effects on the progress and metastasis of cervical cancer were analyzed using cell culture and animal models. Results MiR-154-5p showed low expression in cervical cancer cells. Overexpression of miR-154-5p could markedly inhibit the proliferation, migration, and colony formation ability of SiHa cells, concomitantly leading to G1 arrest of the cell cycle, while silencing miR-154-5p triggered the opposite results. Meanwhile, overexpression of miR-154-5p restrained the growth and metastasis of cervical cancer by silencing CUL2 in vivo. Additionally, miR-154-5p reduced CUL2 level, and overexpression of CUL2 influenced the effect of miR-154-5p in cervical cancer. In conclusion, miR-154-5p restrained the growth and metastasis of cervical cancer by directly silencing CUL2.
