Effects of genotype on hematological and serum biochemical responses of turkey hens to stress

Abstract

Stress accompanying animal production is both an indicator of welfare and a factor that affects economic parameters. Dynamic development of alternative poultry production systems such as outdoor, free range or ecological, strives to achieve best-quality products and to improve the living conditions of birds. Apart from the type and intensity of the external stimuli, the response to stress is determined genetically. Poultry rearing in the aforementioned systems requires selecting the appropriate genotype of birds, often including crosses of fast-growing and slow-growing lines. The objective of this study was to demonstrate differences in the physiological response to stress of fast-growing (FG) and slow-growing (SG) turkeys and their crosses: SF (SG × FG) and FS (FG × SG), based on hematological and biochemical blood analyses. Blood was sampled from 30 turkey hens from each of four genotypes, aged 14 weeks, subjected to standard pre-slaughter handling procedures. Compared to FG and FS turkeys, the SG and SF birds had a high total protein concentration, a higher heterophil (H) ∕ lymphocyte (L) ratio and a higher concentration of cortisol. In turn, the FG turkeys had the highest activity of enzymes: alanine transaminase, aspartate transaminase, lactate dehydrogenase, creatine kinase. Conversely, the FS turkeys had the highest concentrations of total cholesterol and low-density lipoprotein (LDLC) and the lowest concentration of cortisol. Significant positive effects of crossing were reported for total protein, cholinesterase and cortisol. Effects of crossbreeding on the percentage of H, concentrations of total cholesterol, and LDLC and activity of aspartate transaminase in blood of the crosses were significant but negative and more dependent on the sire line. Except for albumins, whose concentration in blood of crosses was higher compared to birds of pure lines, there were no advantages of heterosis. Negative heterosis was estimated for creatinine, the enzymes alanine transaminase and lactate dehydrogenase, and for cortisol. The most similar regarding creatinine, bilirubin, albumins, total protein, H, L and H ∕ L were the FS and SF groups. The greatest differences were in the following parameters: triglycerides, total cholesterol, high-density lipoprotein (HDLC), LDLC, glucose and lactate, which occurred between the pure lines of turkeys. Study results demonstrate various responses to stress in the analyzed groups of turkeys, with the level of stress measured with hematological and biological blood markers being mostly determined by additive genetic variation, and to a lesser extent by the maternal and sire effects.