Fermentation broth from fruit and vegetable waste works: Reducing the risk of human bacterial pathogens in soil by inhibiting quorum sensing

Lin Zhu; Jingpeng Li; Jian Yang; Xiaodi Li; Da Lin; Meizhen Wang

Environment International (Jun 2024)

Fermentation broth from fruit and vegetable waste works: Reducing the risk of human bacterial pathogens in soil by inhibiting quorum sensing

Lin Zhu,
Jingpeng Li,
Jian Yang,
Xiaodi Li,
Da Lin,
Meizhen Wang

Affiliations

Lin Zhu: Science and Technology Cooperation Platform for Low-Carbon Recycling of Waste and Green Development, School of Environmental Science and Engineering, Zhejiang Gongshang University, Hangzhou 310012, Zhejiang, China; Provincial Key Laboratory of Solid Waste Treatment and Recycling, School of Environmental Science and Engineering, Zhejiang Gongshang University, Hangzhou 310012, China
Jingpeng Li: Science and Technology Cooperation Platform for Low-Carbon Recycling of Waste and Green Development, School of Environmental Science and Engineering, Zhejiang Gongshang University, Hangzhou 310012, Zhejiang, China; Provincial Key Laboratory of Solid Waste Treatment and Recycling, School of Environmental Science and Engineering, Zhejiang Gongshang University, Hangzhou 310012, China
Jian Yang: Science and Technology Cooperation Platform for Low-Carbon Recycling of Waste and Green Development, School of Environmental Science and Engineering, Zhejiang Gongshang University, Hangzhou 310012, Zhejiang, China; Provincial Key Laboratory of Solid Waste Treatment and Recycling, School of Environmental Science and Engineering, Zhejiang Gongshang University, Hangzhou 310012, China
Xiaodi Li: Science and Technology Cooperation Platform for Low-Carbon Recycling of Waste and Green Development, School of Environmental Science and Engineering, Zhejiang Gongshang University, Hangzhou 310012, Zhejiang, China; Provincial Key Laboratory of Solid Waste Treatment and Recycling, School of Environmental Science and Engineering, Zhejiang Gongshang University, Hangzhou 310012, China
Da Lin: Science and Technology Cooperation Platform for Low-Carbon Recycling of Waste and Green Development, School of Environmental Science and Engineering, Zhejiang Gongshang University, Hangzhou 310012, Zhejiang, China; Provincial Key Laboratory of Solid Waste Treatment and Recycling, School of Environmental Science and Engineering, Zhejiang Gongshang University, Hangzhou 310012, China
Meizhen Wang: School of Environmental Science and Engineering, Zhejiang Gongshang University, Hangzhou 310012, Zhejiang, China.; Science and Technology Cooperation Platform for Low-Carbon Recycling of Waste and Green Development, School of Environmental Science and Engineering, Zhejiang Gongshang University, Hangzhou 310012, Zhejiang, China; Provincial Key Laboratory of Solid Waste Treatment and Recycling, School of Environmental Science and Engineering, Zhejiang Gongshang University, Hangzhou 310012, China

Journal volume & issue: Vol. 188
p. 108753

Abstract

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Fermentation broth from fruit and vegetable waste (FFVW) has demonstrated remarkable ability as a soil amendment and in reducing antibiotic resistance genes (ARGs) pollution. However, the potential of FFVW to mitigate other microbial contamination such as human bacterial pathogens (HBPs) and virulence factor genes (VFGs), which are closely associated with human health, remains unknown. In this study, metagenomic analysis revealed that FFVW reduced the HBPs with high-risk of ARGs and VFGs including Klebsiella pneumoniae (reduced by 40.4 %), Mycobacterium tuberculosis (reduced by 21.4 %) and Streptococcus pneumoniae (reduced by 38.7 %). Correspondingly, VFG abundance in soil decreased from 3.40 copies/cell to 2.99 copies/cell. Further analysis illustrated that these was mainly attributed to the inhibition of quorum sensing (QS). FFVW reduced the abundance of QS signals, QS synthesis genes such as rpaI and luxS, as well as receptor genes such as rpfC and fusK, resulting in a decreased in risk of ARGs and VFGs. The pure culture experiment revealed that the expression of genes related to QS, VFGs, ARGs and mobile genetic elements (MGEs) were downregulated in Pseudomonas aeruginosa, Staphylococcus aureus, Escherichia coli and K. pneumoniae treated by FFVW, consistent with the result of metagenomic analysis. This study suggested an environmentally friendly approach for controlling soil VFGs/ARGs-carrying HBPs, which is crucial for both soil and human health under the framework of “One Health”.

Published in Environment International

ISSN: 0160-4120 (Print)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Geography. Anthropology. Recreation: Environmental sciences
Website: https://www.journals.elsevier.com/environment-international

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