Evaluation of Curcumin Nano-micelle on Proliferation and Apoptosis of HT29 and Hct116 Colon Cancer Cell Lines

Abstract

Background: Curcumin is a natural polyphenolic material with antioxidative, anti-inflammatory, and anticancer effects. In this study, we attempted to assay antiproliferative and apoptotic properties of polymeric micelles of curcumin on two colorectal cancer cell lines and normal human fibroblast cells. Method: In this experimental study, cancer cells HT29, HCT116 and normal human fibroblast cells (HGF) were subjected to concentrations of Nano- curcumin (1, 50, 100, 250, and 500 μg/ml). After incubation for 48 hours, cell viability was assessed with "MTT"(3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) assay. Annexin V-FITC and Propidium iodide staining were done with flow cytometry for evaluation of apoptosis. The results were shown as mean ± standard deviation. Statistical significance was assessed utilizing ANOVA and Dunnetts t-test (P < 0.01). Results: According to MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay results, IC50 value of Nano- curcumin in HT29, HCT116, and HGF were 70.63, 123.9, and 168.53 μg/ml, respectively. We also discovered that Nano-curcumin can make indicative apoptosis in cancer cells, which could be compared with cisplatin <0.01. Conclusion: These results revealed remarkable antiproliferative and apoptotic effects of polymeric Nano-micelles of curcumin in colorectal cancer cell lines.

Keywords

• colonic neoplasms
• cell proliferation
• apoptosis
• curcumin