Diabetes, Metabolic Syndrome and Obesity (Oct 2021)
LncRNA LINC00673 is Downregulated in Diabetic Retinopathy and Regulates the Apoptosis of Retinal Pigment Epithelial Cells via Negatively Regulating p53
Abstract
Yan Cheng, Yanxia Zhu, Linli Ma Department of Ophthalmology, The Second People’s Hospital of Lianyungang, Lianyungang 222000, Jiangsu Province, People’s Republic of ChinaCorrespondence: Linli MaDepartment of Ophthalmology, The Second People’s Hospital of Lianyungang, Lianyungang 222000, Jiangsu Province, People’s Republic of ChinaEmail [email protected]: Long noncoding RNA (LncRNA) LINC00673 has been proven to play critical roles in cancer biology, while its role in other diseases is unknown. It has been reported that LINC00673 could interact with p53, a critical player in diabetes and diabetic complications, suggesting that LINC00673 may also participate in diabetic retinopathy (DR). This study aimed to investigate the role of LINC00673 in DR.Methods: The present study included 3 groups of participants, including DR group, diabetes (DB) group, and healthy control (Control) group. Flow cytometry was utilized to determine cell apoptosis. Proteins and messenger RNAs (mRNAs) were estimated by Western blot and quantitative reverse transcription PCR (qRT-PCR), respectively.Results: LINC00673 was downregulated in plasma samples of DR patients (n=60) in comparison with the healthy controls (n=60) and negatively correlated with p53 only across DR patients but not across the healthy controls. In retinal pigment epithelial cells (RPECs), high glucose treatment downregulated LINC00673. Moreover, LINC00673 overexpression downregulated p53 and decreased RPEC apoptosis, while LINC00673 silencing upregulated p53 and increased RPEC apoptosis. In addition, p53 overexpression reduced the effects of LINC00673 overexpression.Conclusion: LINC00673 is downregulated in DR patients and regulates RPEC apoptosis via negatively regulating p53.Keywords: diabetic retinopathy, LINC00673, p53, apoptosis
