Phyto-Mediated Synthesis of Silver Nanoparticles Using <i>Terminalia chebula</i> Fruit Extract and Evaluation of Its Cytotoxic and Antimicrobial Potential
Veena Malligere Ankegowda,
Shiva Prasad Kollur,
Shashanka K. Prasad,
Sushma Pradeep,
Chandan Dhramashekara,
Anisha S. Jain,
Ashwini Prasad,
Chandrashekar Srinivasa,
Poojitha B. Sridhara Setty,
S. M. Gopinath,
Rajendra Prasad S.,
Ali H. Bahkali,
Asad Syed,
Chandan Shivamallu
Affiliations
Veena Malligere Ankegowda
Department of Chemistry, Bangalore Institute of Technology, K.R. Road, V.V. Puram, Karnataka, Bangalore 560 004, India
Shiva Prasad Kollur
Department of Sciences, Amrita School of Arts and Sciences, Amrita Vishwa Vidyapeetham, Mysuru Campus, Karnataka 570 026, India
Shashanka K. Prasad
Department of Biotechnology and Bioinformatics, School of Life Sciences, JSS Academy of Higher Education and Research, Mysuru, Karnataka 570 015, India
Sushma Pradeep
Department of Biotechnology and Bioinformatics, School of Life Sciences, JSS Academy of Higher Education and Research, Mysuru, Karnataka 570 015, India
Chandan Dhramashekara
Department of Biotechnology and Bioinformatics, School of Life Sciences, JSS Academy of Higher Education and Research, Mysuru, Karnataka 570 015, India
Anisha S. Jain
Department of Microbiology and Tissue Culture, School of Life Sciences, JSS Academy of Higher Education and Research, Mysuru, Karnataka 570 015, India
Ashwini Prasad
Department of Microbiology and Tissue Culture, School of Life Sciences, JSS Academy of Higher Education and Research, Mysuru, Karnataka 570 015, India
Chandrashekar Srinivasa
Department of Biotechnology, Davangere University, Shivagangotri, Davangere, Karnataka 577 007, India
Poojitha B. Sridhara Setty
Department of Biotechnology, Davangere University, Shivagangotri, Davangere, Karnataka 577 007, India
S. M. Gopinath
Department of Biotechnology, Davangere University, Shivagangotri, Davangere, Karnataka 577 007, India
Rajendra Prasad S.
Department of Chemistry, Davangere University, Shivagangotri, Davangere, Karnataka 577 007, India
Ali H. Bahkali
Department of Botany and Microbiology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia
Asad Syed
Department of Botany and Microbiology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia
Chandan Shivamallu
Department of Biotechnology and Bioinformatics, School of Life Sciences, JSS Academy of Higher Education and Research, Mysuru, Karnataka 570 015, India
The increasing interest in developing potent non-toxic drugs in medicine is widening the opportunities for studying the usage of nanostructures in the treatment of various diseases. The present work reports a method for a facile and an eco-friendly synthesis of silver nanoparticles (AgNPs) using Terminalia chebula fruit extract (TCE). The obtained AgNPs was characterized by using different spectroscopic and microscopic techniques. The analysis of the results revealed that the as-obtained AgNPs have spherical morphology with an average diameter of 22 nm. Furthermore, the preliminary bioactivity evaluations revealed that the bio-conjugation of AgNPs, using TCE, significantly enhanced the antibacterial and anti-breast cancer potentials of the latter. The antibacterial activity of the as-prepared AgNPs showed that B. subtilis was more sensitive towards the AgNPs, followed by P. aeruginosa; while, E. coli and S. mutans showed comparatively minimal sensitivity toward the AgNPs. The IC50 values of TCE, AgNPs and TCE + AgNPs treatment of MCF-7 were found to be 17.53, 14.25 and 6.484 µg/mL, respectively. Therefore, it can be ascertained that the bio-conjugation may provide a headway with regard to the therapeutic employment of T. chebula, upon mechanistically understanding the basis of observed antibacterial and anticancer activities.
