Effects of Laser Application on Alveolar Bone Mesenchymal Stem Cells and Osteoblasts: An In Vitro Study
Luminița Lazăr,
Doina Ramona Manu,
Timea Dako,
Maria-Alexandra Mârțu,
Mircea Suciu,
Alina Ormenișan,
Mariana Păcurar,
Ana-Petra Lazăr
Affiliations
Luminița Lazăr
Department of Periodontology, George Emil Palade University of Medicine, Pharmacy, Science, and Technology of Târgu Mures, 38 Ghe. Marinescu Street, 540139 Târgu Mures, Romania
Doina Ramona Manu
Center for Advanced Medical and Pharmaceutical Research, George Emil Palade University of Medicine, Pharmacy, Science, and Technology of Targu Mures, 540142 Târgu Mures, Romania
Timea Dako
Department of Odontology and Oral Pathology, George Emil Palade University of Medicine, Pharmacy, Science, and Technology of Târgu Mures, 38 Ghe. Marinescu Street, 540139 Târgu Mures, Romania
Maria-Alexandra Mârțu
Department of Periodontology, Grigore T. Popa University of Medicine and Pharmacy Iasi, Universitatii Street 16, 700115 Iasi, Romania
Mircea Suciu
Department of Oral Rehabilitation and Occlusology, George Emil Palade University of Medicine, Pharmacy, Science, and Technology of Târgu Mures, 38 Ghe. Marinescu Street, 540139 Târgu Mures, Romania
Alina Ormenișan
Department of Oral and Maxillofacial Surgery, George Emil Palade University of Medicine, Pharmacy, Science, and Technology of Târgu Mures, 38 Ghe. Marinescu Street, 540139 Târgu Mures, Romania
Mariana Păcurar
Department of Orthodontics, George Emil Palade University of Medicine, Pharmacy, Science, and Technology of Târgu Mures, 38 Ghe. Marinescu Street, 540139 Târgu Mures, Romania
Ana-Petra Lazăr
Institution Organizing University Doctoral Studies (I. O. S. U. D.), George Emil Palade University of Medicine, Pharmacy, Sciences and Technology of Târgu Mureş, 38 Ghe. Marinescu Street, 540139 Târgu Mures, Romania
Mesenchymal stem cells isolated from the bone marrow have a great differentiation potential, being able to produce many cell lines, including osteoblasts. Osteoblasts have an important role in bone remodeling by actively participating in the maturation and mineralization of the extracellular matrix. The aim of this study was to determine the effect of laser application on the viability and proliferation of osteoblasts. Methods: Alveolar bone was harvested from 8 patients and placed into a culture medium to induce proliferation of mesenchymal stem cells. These were differentiated into osteoblasts in special conditions. The cells from each patient were split into two groups, one was treated using a 980 nm laser (1W output power, pulsed mode, 20 s, 50 mm distance) (laser “+”) and the other one did not receive laser stimulation (laser “-”). Results: Using the confocal microscope, we determined that the cells from the laser “+” group were more active when compared to the laser “-” group. The number of cells in the laser “+” group was significantly greater compared to the laser “-” group as the ImageJ-NIH software showed (p = 0.0072). Conclusions: Laser application increases the proliferation rate of osteoblasts and intensifies their cellular activity.
