Discovery of Three New Monoterpenoid Indole Alkaloids from the Leaves of <i>Gardneria multiflora</i> and Their Vasorelaxant and AChE Inhibitory Activities
Sheng-Yuan Zhang,
Zi-Wei Li,
Jie Xu,
Qiu-Ling Chen,
Min Song,
Qing-Wen Zhang
Affiliations
Sheng-Yuan Zhang
Guangdong Provincial Key Laboratory of Conservation and Precision Utilization of Characteristic Agricultural Resources in Mountainous Areas, Jiaying University, Meizhou 514015, China
Zi-Wei Li
NMPA Key Laboratory for Quality Evaluation of TCM, Guangdong Provincial Engineering Research Center for Modernization of TCM, Jinan University, Guangzhou 510632, China
Jie Xu
NMPA Key Laboratory for Quality Evaluation of TCM, Guangdong Provincial Engineering Research Center for Modernization of TCM, Jinan University, Guangzhou 510632, China
Qiu-Ling Chen
NMPA Key Laboratory for Quality Evaluation of TCM, Guangdong Provincial Engineering Research Center for Modernization of TCM, Jinan University, Guangzhou 510632, China
Min Song
State Key Laboratory of Quality Research in Chinese Medicine and Institute of Chinese Medical Sciences, University of Macau, Macau 999078, China
Qing-Wen Zhang
State Key Laboratory of Quality Research in Chinese Medicine and Institute of Chinese Medical Sciences, University of Macau, Macau 999078, China
Three novel monoterpenoid indole alkaloids gardflorine A (1), gardflorine B (2), and gardflorine C (3) were isolated from the leaves of Gardneria multiflora. Their structures, including absolute configurations, were established on the basis of spectroscopic methods (MS, UV, IR, 1D and 2D NMR) and circular dichroism experiments. All the compounds were evaluated for their vasorelaxant and acetylcholinesterase (AChE) inhibitory activities. Compound 1 exhibited potent vasorelaxant activity, with an EC50 value of 8.7 μM, and compounds 2 and 3 showed moderate acetylcholinesterase (AChE) inhibitory activities, with IC50 values of 26.8 and 29.2 μM, respectively.
