Oxygen-transport function of hemoglobin in rats under acute and chronic intoxication chlorpyrifos used in various doses

Abstract

The paper is dedicated to the results of investigation of the impact of widely used pesticide chlorpyrifos on the oxygen-transport function of hemoglobin in rats. Сhlorpyrifos was administered orally to experimental animals in the form of oil solution. The rats were subjected to acute (30 and 70 mg/kg) and chronic (5, 10 and 15 mg/kg) poisoning. It was revealed that chronic admi­nistration of chlorpyrifos (daily for 30 days) at all applied doses led to a reducing of hemoglobin’s affinity to oxygen, that was manifested as a right shift of the oxygen – hemoglobin dissociation curve. In turn, a single injection of chlorpyrifos in 30 mg/kg resulted in statistically significant changes in the dynamics of oxygen saturation of hemoglobin only at 10th day of the experiment, while at the first, third and sixth day after poisoning, no significant effects were observed. We also found that the acute intoxication with chlorpyrifos in 70 mg/kg led to a decrease in the hemoglobin’s affinity for oxygen, which was manifested as a shift in the oxyhemoglobin dissociation curve. This trend was observed both in rats treated with only oil solution of chlorpyrifos and in animals with received the pesticide in a combination with vitamins A and E.

Keywords

• rats
• poisoning
• сhlorpyrifos
• acetylcholinesterase
• hemoglobin