Diversity of Anal HPV and Non-HPV Sexually Transmitted Infections and Concordance with Genital Infections in HIV-Infected and HIV-Uninfected Women in the Tapajós Region, Amazon, Brazil
Luana Lorena Silva Rodrigues,
José Henrique Pilotto,
Katrini Guidolini Martinelli,
Alcina F. Nicol,
Vanessa Salete De Paula,
Tarik Gheit,
Nathália Silva Carlos Oliveira,
Carlos Silva-de-Jesus,
Vikrant V. Sahasrabuddhe,
Diane M. Da Silva,
W. Martin Kast,
Justin Hardick,
Charlotte A. Gaydos,
Mariza Gonçalves Morgado
Affiliations
Luana Lorena Silva Rodrigues
Programa de Pós-Graduação em Ciências da Saúde, Instituto de Saúde Coletiva, Universidade Federal do Oeste do Pará, Santarém 68135-110, Brazil
José Henrique Pilotto
Laboratório de AIDS e Imunologia Molecular, IOC-FIOCRUZ, Rio de Janeiro 21040-360, Brazil
Katrini Guidolini Martinelli
Departamento de Medicina Social, Universidade Federal do Espírito Santo, Vitória 29075-910, Brazil
Alcina F. Nicol
Instituto Nacional de Infectologia Evandro Chagas, INI-FIOCRUZ, Rio de Janeiro 21040-360, Brazil
Vanessa Salete De Paula
Laboratório de Virologia Molecular e Parasitologia, IOC-FIOCRUZ, Rio de Janeiro 21040-360, Brazil
Tarik Gheit
International Agency for Research on Cancer, 69366 Lyon, France
Nathália Silva Carlos Oliveira
Departamento de Patologia, Faculdade de Medicina, Universidade Federal Fluminense, Rio de Janeiro 23033-900, Brazil
Carlos Silva-de-Jesus
Laboratório de AIDS e Imunologia Molecular, IOC-FIOCRUZ, Rio de Janeiro 21040-360, Brazil
Vikrant V. Sahasrabuddhe
Division of Cancer Prevention, National Cancer Institute, Bethesda, MD 20892, USA
Diane M. Da Silva
Department of Obstetrics and Gynecology, University of Southern California, Los Angeles, CA 90033, USA
W. Martin Kast
Department of Obstetrics and Gynecology, University of Southern California, Los Angeles, CA 90033, USA
Justin Hardick
Division of Infectious Diseases, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
Charlotte A. Gaydos
Division of Infectious Diseases, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
Mariza Gonçalves Morgado
Laboratório de AIDS e Imunologia Molecular, IOC-FIOCRUZ, Rio de Janeiro 21040-360, Brazil
The aim of this study was to classify the diversity of anal HPV and non-HPV sexually transmitted infections (STIs) and compare the concordance between anal and genital infections in HIV-infected and uninfected women living in the Tapajós region, Amazon, Brazil. A cross-sectional study was performed with 112 HIV-uninfected and 41 HIV-infected nonindigenous women. Anal and cervical scrapings were collected and analyzed for HPV, Chlamydia trachomatis (CT), Neisseria gonorrheae (NG), Trichomonas vaginalis (TV), Mycoplasma genitalium (MG), and Human alphaherpesvirus 2 (HSV-2). The Kappa test evaluated the concordance between anal and genital infections. The overall prevalence of anal HPV infection was 31.3% in HIV-uninfected and 97.6% in HIV-infected women. The most frequent anal high-risk HPV (hrHPV) types were HPV18 and HPV16 in HIV-uninfected women and HPV51, HPV59, HPV31, and HPV58 in HIV-infected women. Anal HPV75 Betapapillomavirus was also identified. Anal non-HPV STIs were identified in 13.0% of all participants. The concordance analysis was fair for CT, MG, and HSV-2, almost perfect agreement for NG, moderate for HPV, and variable for the most frequent anal hrHPV types. Thus, a high prevalence of anal HPV infection with moderate and fair concordance between anal and genital HPV and non-HPV STIs was observed in our study.
