CryoEM structure of the human SLC4A4 sodium-coupled acid-base transporter NBCe1

Kevin W. Huynh; Jiansen Jiang; Natalia Abuladze; Kirill Tsirulnikov; Liyo Kao; Xuesi Shao; Debra Newman; Rustam Azimov; Alexander Pushkin; Z. Hong Zhou; Ira Kurtz

doi:10.1038/s41467-018-03271-3

Nature Communications (Mar 2018)

CryoEM structure of the human SLC4A4 sodium-coupled acid-base transporter NBCe1

Kevin W. Huynh,
Jiansen Jiang,
Natalia Abuladze,
Kirill Tsirulnikov,
Liyo Kao,
Xuesi Shao,
Debra Newman,
Rustam Azimov,
Alexander Pushkin,
Z. Hong Zhou,
Ira Kurtz

Affiliations

Kevin W. Huynh: Department of Medicine, Division of Nephrology, David Geffen School of Medicine, University of California
Jiansen Jiang: California NanoSystems Institute, University of California
Natalia Abuladze: Department of Medicine, Division of Nephrology, David Geffen School of Medicine, University of California
Kirill Tsirulnikov: Department of Medicine, Division of Nephrology, David Geffen School of Medicine, University of California
Liyo Kao: Department of Medicine, Division of Nephrology, David Geffen School of Medicine, University of California
Xuesi Shao: Department of Neurobiology, University of California
Debra Newman: Department of Medicine, Division of Nephrology, David Geffen School of Medicine, University of California
Rustam Azimov: Department of Medicine, Division of Nephrology, David Geffen School of Medicine, University of California
Alexander Pushkin: Department of Medicine, Division of Nephrology, David Geffen School of Medicine, University of California
Z. Hong Zhou: California NanoSystems Institute, University of California
Ira Kurtz: Department of Medicine, Division of Nephrology, David Geffen School of Medicine, University of California

DOI: https://doi.org/10.1038/s41467-018-03271-3
Journal volume & issue: Vol. 9, no. 1
pp. 1 – 9

Abstract

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Na+-coupled acid-base membrane transport proteins regulate blood pressure, ion homeostasis and acid-base chemistry. Here the authors present the 3.9 Å resolution cryoEM structure of the sodium-bicarbonate cotransporter NBCe1 and characterize its ion coordination site and ion accessibility pathway with mutagenesis experiments.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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