Bio-clickable mussel-inspired peptides improve titanium-based material osseointegration synergistically with immunopolarization-regulation

Jie Sun; Yingkang Huang; Huan Zhao; Junjie Niu; Xuwei Ling; Can Zhu; Lin Wang; Huilin Yang; Zhilu Yang; Guoqing Pan; Qin Shi

Bioactive Materials (Mar 2022)

Bio-clickable mussel-inspired peptides improve titanium-based material osseointegration synergistically with immunopolarization-regulation

Jie Sun,
Yingkang Huang,
Huan Zhao,
Junjie Niu,
Xuwei Ling,
Can Zhu,
Lin Wang,
Huilin Yang,
Zhilu Yang,
Guoqing Pan,
Qin Shi

Affiliations

Jie Sun: Department of Orthopaedics, The First Affiliated Hospital of Soochow University, Orthopaedic Institute of Soochow University, 899 Pinghai, Suzhou, Jiangsu, 215031, China
Yingkang Huang: Department of Orthopaedics, The First Affiliated Hospital of Soochow University, Orthopaedic Institute of Soochow University, 899 Pinghai, Suzhou, Jiangsu, 215031, China
Huan Zhao: Department of Orthopaedics, The First Affiliated Hospital of Soochow University, Orthopaedic Institute of Soochow University, 899 Pinghai, Suzhou, Jiangsu, 215031, China
Junjie Niu: Department of Orthopaedics, The First Affiliated Hospital of Soochow University, Orthopaedic Institute of Soochow University, 899 Pinghai, Suzhou, Jiangsu, 215031, China
Xuwei Ling: Department of Orthopaedics, The First Affiliated Hospital of Soochow University, Orthopaedic Institute of Soochow University, 899 Pinghai, Suzhou, Jiangsu, 215031, China
Can Zhu: Department of Orthopaedics, The First Affiliated Hospital of Soochow University, Orthopaedic Institute of Soochow University, 899 Pinghai, Suzhou, Jiangsu, 215031, China
Lin Wang: Department of Orthopaedics, The First Affiliated Hospital of Soochow University, Orthopaedic Institute of Soochow University, 899 Pinghai, Suzhou, Jiangsu, 215031, China
Huilin Yang: Department of Orthopaedics, The First Affiliated Hospital of Soochow University, Orthopaedic Institute of Soochow University, 899 Pinghai, Suzhou, Jiangsu, 215031, China
Zhilu Yang: Affiliated Dongguan Hospital, Southern Medical University, No. 3 Wandao Road, Dongguan, Guangdong, 523059, China; Guangdong Provincial Key Laboratory of Shock and Microcirculation, No. 1023 Shatai Road, Guangzhou, Guangdong, 510080, China; Corresponding author. Affiliated Dongguan Hospital, Southern Medical University, No. 3 Wandao Road, Dongguan, Guangdong, 523059, China.
Guoqing Pan: Institute for Advanced Materials, School of Materials Science and Engineering, Jiangsu University, 301 Xuefu Road, Zhenjiang, Jiangsu, 212013, China; Corresponding author.
Qin Shi: Department of Orthopaedics, The First Affiliated Hospital of Soochow University, Orthopaedic Institute of Soochow University, 899 Pinghai, Suzhou, Jiangsu, 215031, China; Corresponding author.

Journal volume & issue: Vol. 9
pp. 1 – 14

Abstract

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Upon the osteoporotic condition, sluggish osteogenesis, excessive bone resorption, and chronic inflammation make the osseointegration of bioinert titanium (Ti) implants with surrounding bone tissues difficult, often lead to prosthesis loosening, bone collapse, and implant failure. In this study, we firstly designed clickable mussel-inspired peptides (DOPA-N3) and grafted them onto the surfaces of Ti materials through robust catechol-TiO2 coordinative interactions. Then, two dibenzylcyclooctyne (DBCO)-capped bioactive peptides RGD and BMP-2 bioactive domain (BMP-2) were clicked onto the DOPA-N3-coated Ti material surfaces via bio-orthogonal reaction. We characterized the surface morphology and biocompatibility of the Ti substrates and optimized the osteogenic capacity of Ti surfaces through adjusting the ideal ratios of BMP-2/RGD at 3:1. In vitro, the dual-functionalized Ti substrates exhibited excellent promotion on adhesion and osteogenesis of mesenchymal stem cells (MSCs), and conspicuous immunopolarization-regulation to shift macrophages to alternative (M2) phenotypes and inhibit inflammation, as well as enhancement of osseointegration and mechanical stability in osteoporotic rats. In summary, our biomimetic surface modification strategy by bio-orthogonal reaction provided a convenient and feasible method to resolve the bioinertia and clinical complications of Ti-based implants, which was conducive to the long-term success of Ti implants, especially in the osteoporotic or inflammatory conditions.

Published in Bioactive Materials

ISSN: 2452-199X (Online)
Publisher: KeAi Communications Co., Ltd.
Country of publisher: China
LCC subjects: Technology: Electrical engineering. Electronics. Nuclear engineering: Materials of engineering and construction. Mechanics of materials; Science: Biology (General)
Website: https://www.keaipublishing.com/en/journals/bioactive-materials/

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