Modificações do volume e da histologia de focos de endometriose em ratas tratadas com sinvastatina Changes in the volume and histology of focus of endometriosis in rats treated with sinvastatin

Abstract

OBJETIVO: analisar alterações macroscópicas e histológicas que ocorrem com o uso da sinvastatina em endometriose experimental em ratas. MÉTODOS: quarenta ratas da linhagem Wistar foram submetidas à técnica de autotransplante uterino em mesentério. Após três semanas, 24 ratas desenvolveram endometriose experimental grau III e foram divididas em dois grupos: Sinvastatina (dado 20 mg/kg/dia via oral) e Controle (dado cloreto de sódio a 0,9% na quantidade de 1 mL/100 g de peso corpóreo via oral), que receberam gavagem durante 14 dias seguido de morte. Os volumes dos implantes foram calculados [4pi (comprimento/2) x (largura/2) x (altura/2)/3] nas intervenções cirúrgicas e após a morte dos animais. Os autotransplantes foram retirados, corados com a hematoxilina-eosina e analisados à microscopia de luz. Foram usados o teste de Mann-Whitney para amostras independentes e o teste de Wilcoxon para amostras relacionadas. Para avaliação histológica, foi usado o teste exato de Fisher, adotando-se nível de significância de 5%. RESULTADOS: a diferença entre os volumes médios iniciais dos autotransplantes nos dois grupos foi insignificante (p=1,00), e, entre os volumes médios finais, significante (p=0,04). Houve aumento significativo (p=0,01) entre os volumes médios iniciais e finais do Grupo Controle e redução insignificante no Grupo Sinvastatina (p=0,95). Histologicamente (p=0,64), o Grupo Sinvastatina (n=9) mostrou a parede epitelial moderadamente preservada em sete casos (77,80%) e dois casos com camada epitelial bem preservada (22,2%), e o Grupo Controle (n=12) com sete casos (58,30%) moderadamente preservados e cinco casos (41,70%) bem preservados. CONCLUSÕES: a sinvastatina impediu o crescimento dos focos de endometriose experimental. São promissores os estudos com uso da sinvastatina por período mais prolongado.PURPOSE: to analyze the macroscopic and histological changes that occur with the use of sinvastatin in experimental endometriosis in female rats. METHODS: forty Wistar female rats were submitted to the technique of uterine self-transplant in mesenterium. After three weeks, 24 of them developed experimental endometriosis grade III, and were divided in two groups: one group received sinvastatin orally (20 mg/kg/day) and the other (control group) received 0.9% of sodium chloride orally (1 mL/100 g of body weight/day). Both groups received gavage for 14 days, followed by death. The implant volume was calculated [4pi (lenght/2) x (width/2) x (height/2)/3] at the surgical intervention and after the animal’s death. The self-transplants were removed, dyed with hematoxylin-eosin and analyzed by light microscopy. The Mann-Whitney’s test was used in the independent samples and the Wilcoxon’s test for the related samples. The Fisher’s exact test was used for the histological evaluation, with a significance level of 5%. RESULTS: the difference between groups of the initial average volumes of the self-transplants was not significant (p=1.00), but became significant for the final average volumes (p=0.04). There was a significant increase (p=0.01) between the initial and final average volumes in the control group, and a no significant decrease in the sinvastatin group (p=0.95). Histologically, the sinvastatin group (n=9) presented seven cases (77.8%) of moderately preserved and two cases (22.2%) of well preserved epithelial wall, while the control group (n=12) presented seven cases (58.3%) of moderately preserved and five cases (41.7%) of well preserved epithelial wall. CONCLUSIONS: sinvastatin prevented the growth of experimental endometriosis. Studies with sinvastatin for longer periods are promising.

Keywords

• Sinvastatina
• Endometriose
• Ratos Wistar
• Simvastatin
• Endometriosis
• Rats Wistar