A141

Abstract

The aim of this study was to investigate the quantitative changes in the phospholipid (PL) content of peripheral blood mononuclear cells (MNC), plasma membrane (PM), fraction in breast (BC) and cervical cancers (CC) compared to normal levels. Eight PL fractions were identified by TLC method in the PM of MNC, namely: lysophosphatidylcholines (LPC), sphingomyelins (SPM), phosphatidylcholines (PC), phosphatidylinositols (PI), phosphatidylserines (PS), phosphatidylethanolamines (PE), phosphatidic acids (PA) and diphosphatidylglycerols (DPG). Data obtained indicate that all PLs, quantified in this study, were significantly altered in blood MNC of cancer patients compared to healthy individuals. It was shown that compared to norm levels of LPC, PC, PE fractions were reliable increased in BC and CC, when PI, PS, PA – decreased. Notably, regular disturbances reveled in BC and CC were identical with those observed earlier in chronic lymphocytic leukemia and also distinctly individual for each patient. We conclude that alterations in PLs content of crude MNC PMs have been associated with disease pathology and similarly involved in the onset and evolution of diverse forms of cancer. These data can be useful for prospective biomarkers selection and cancer definition as well as for discovery of new personalized treatment modes.