PLoS ONE (Jan 2014)

A recombinant avian leukosis virus subgroup j for directly monitoring viral infection and the selection of neutralizing antibodies.

  • Qi Wang,
  • Xiaofei Li,
  • Xiaolin Ji,
  • Jingfei Wang,
  • Nan Shen,
  • Yulong Gao,
  • Xiaole Qi,
  • Yongqiang Wang,
  • Honglei Gao,
  • Shide Zhang,
  • Xiaomei Wang

DOI
https://doi.org/10.1371/journal.pone.0115422
Journal volume & issue
Vol. 9, no. 12
p. e115422

Abstract

Read online

Avian leukosis virus subgroup J (ALV-J) has induced serious clinical outbreaks and has become a serious infectious disease of chickens in China. We describe here the creation of a recombinant ALV-J tagged with the enhanced green fluorescent protein (named rHPRS-103EGFP). We successfully utilize the rHPRS-103EGFP to visualize viral infection and for development of a simplified serum-neutralization test.