Проблемы особо опасных инфекций (Dec 2017)

Circulation of Lyssaviruses (Lyssavirus) among the Small Mammals in the Territory of the Republic of Guinea

  • A. M. Porshakov,
  • Yu. V. Kononova,
  • V. A. Ternovoy,
  • A. V. Zaikovskaya,
  • K. A. Nikiforov,
  • D. A. Agafonov,
  • A. O. Sementsova,
  • N. Keita,
  • S. A. Yakovlev,
  • A. E. Nesterov,
  • A. A. Sergeev,
  • A. Toure,
  • S. Boumbaly,
  • P. P. Nikitin,
  • A. A. Lopatin,
  • V. B. Loktev

DOI
https://doi.org/10.21055/0370-1069-2017-4-72-76
Journal volume & issue
Vol. 0, no. 4
pp. 72 – 76

Abstract

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Objective is to study the role of small mammals, habitant in the Republic of Guinea, in Lyssavirus circulation. Materials and methods. Investigations were conducted using RT-PCR; nucleotide sequence of Lyssavirus cDNA fragments was identified with the help of sequencing with further phylogenetic analysis. Results and conclusions. Tested have been 356 brain samples from small mammals for the presence of Lyssavirus RNA using RT-PCR with genus-specific primers. The animals were caught in the suburbs of Kindia city in 2016. The samples were obtained from wild animals pertaining to Rodentia, Chiroptera, Eulipotyphla, and Carnivora orders.Lyssavirus RNA was detected in 31 samples (8.7 %). For 14 PCR positive samples the appurtenance to Lyssavirus was confirmed through identification and analysis of nucleotide sequences of the collected short cDAN fragments of viral genome. The presence of rabies virus RNA in positive tests was excluded from PCR with the help of species specific primers. The pool of samples from black rats, Rattus rattus, positive for Lyssavirus RNA, contained RNA characteristic of Mokola lyssavirus species. Specified has been nucleotide sequence of matrix protein M gene fragment of Mokola virus. Genetic material of Mokola virus was detected in the Republic of Guinea for the first time ever.

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