Results of night-time surface layer turbulence measurements using ESO LuSci at the Maidanak observatory are presented. Turbulence in the surface layer was evaluated at the Maidanak observatory using LuSci during the period of 2021–2022. The overall median DIMM-seeing was 0.74 arcseconds during this period. It was determined that the seeing in the surface layer up to 256 m equals 0.44 arcseconds. This corresponds to 42% of the contribution to the integral seeing in the entire atmosphere. A telescope installed at 6 m above the ground will be affected by 33% of the integral turbulence and about 79% of the surface layer one. Taking into account that the free atmosphere contributes around 30%, we conclude that around 30% of the overall seeing is contributed by the boundary layer above the Maidanak observatory.