Universal calibration for natural polysaccharides in aqueous Size-exclusion chromatography

Abstract

This article shows the validity of the Universal calibration concept in aqueous Size-exclusion chromatography for arabinogalactan and carboxymethylchitosans is presented. The electrostatic effects of these polysaccharides during separation process were eliminated by utilizing of a water-salt eluent as a mobile phase, and the size-exclusion separation mechanism of this method was realized.