The sensitivity of the FLT3-ITD detection method is an important consideration when diagnosing acute myeloid leukemia

Masahiro Sakaguchi; Nana Nakajima; Hiroki Yamaguchi; Yuho Najima; Katsuhiro Shono; Atsushi Marumo; Ikuko Omori; Yusuke Fujiwara; Kazuki Terada; Shunsuke Yui; Satoshi Wakita; Miho Mitaya; Kunihito Arai; Tomoaki Kitano; Noriko Doki; Kazuteru Ohashi; Koiti Inokuchi

Leukemia Research Reports (Jan 2020)

The sensitivity of the FLT3-ITD detection method is an important consideration when diagnosing acute myeloid leukemia

Masahiro Sakaguchi,
Nana Nakajima,
Hiroki Yamaguchi,
Yuho Najima,
Katsuhiro Shono,
Atsushi Marumo,
Ikuko Omori,
Yusuke Fujiwara,
Kazuki Terada,
Shunsuke Yui,
Satoshi Wakita,
Miho Mitaya,
Kunihito Arai,
Tomoaki Kitano,
Noriko Doki,
Kazuteru Ohashi,
Koiti Inokuchi

Affiliations

Masahiro Sakaguchi: Department of Hematology, Nippon Medical School, Tokyo, Japan
Nana Nakajima: Department of Hematology, Nippon Medical School, Tokyo, Japan
Hiroki Yamaguchi: Department of Hematology, Nippon Medical School, Tokyo, Japan; Corresponding author at: Department of Hematology, Nippon Medical School, 1-1-5 Sendagi, Bunkyo-Ku, Tokyo, 113-8603, Japan.
Yuho Najima: Hematology Division, Tokyo Metropolitan Cancer and Infectious Diseases Center, Komagome Hospital, Tokyo, Japan
Katsuhiro Shono: Department of Hematology, Chiba Aoba Municipal Hospital, Chiba, Japan
Atsushi Marumo: Department of Hematology, Nippon Medical School, Tokyo, Japan
Ikuko Omori: Department of Hematology, Nippon Medical School, Tokyo, Japan
Yusuke Fujiwara: Department of Hematology, Nippon Medical School, Tokyo, Japan
Kazuki Terada: Department of Hematology, Nippon Medical School, Tokyo, Japan
Shunsuke Yui: Department of Hematology, Nippon Medical School, Tokyo, Japan
Satoshi Wakita: Department of Hematology, Nippon Medical School, Tokyo, Japan
Miho Mitaya: Department of Hematology, Nippon Medical School, Tokyo, Japan
Kunihito Arai: Department of Hematology, Nippon Medical School, Tokyo, Japan
Tomoaki Kitano: Department of Hematology, Nippon Medical School, Tokyo, Japan
Noriko Doki: Hematology Division, Tokyo Metropolitan Cancer and Infectious Diseases Center, Komagome Hospital, Tokyo, Japan
Kazuteru Ohashi: Hematology Division, Tokyo Metropolitan Cancer and Infectious Diseases Center, Komagome Hospital, Tokyo, Japan
Koiti Inokuchi: Department of Hematology, Nippon Medical School, Tokyo, Japan

Journal volume & issue: Vol. 13
p. 100198

Abstract

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Fms-like tyrosine kinase 3-internal tandem duplication (FLT3-ITD) is a key predictive factor for the prognosis of acute myeloid leukemia (AML). We compared the detection sensitivity of fragment analysis with that of PCR-electrophoresis using MV4-11 (FLT3-ITD) and NKM-1 (FLT3-wild type) cell lines. DNA of these cells was mixed at different ratios and subjected to PCR-electrophoresis or fragment analysis. PCR-electrophoresis was found to have an FLT3-ITD allelic ratio (AR) detection limit of 0.034–0.072. Visual inspection of the PCR-electrophoresis revealed a lower detection sensitivity than that of fragment analysis. Therefore, it is essential to conduct fragment analysis when screening for FLT3-ITD.

Published in Leukemia Research Reports

ISSN: 2213-0489 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Medicine: Internal medicine: Neoplasms. Tumors. Oncology. Including cancer and carcinogens
Website: http://www.journals.elsevier.com/leukemia-research-reports/

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