Mutable Collagenous Tissue Isolated from Echinoderms Leads to the Production of a Dermal Template That Is Biocompatible and Effective for Wound Healing in Rats
Anna Carolo,
Luca Melotti,
Giulia Zivelonghi,
Roberta Sacchetto,
Eylem Emek Akyürek,
Tiziana Martinello,
Andrea Venerando,
Ilaria Iacopetti,
Michela Sugni,
Giordana Martinelli,
Margherita Roncoroni,
Stefania Marzorati,
Silvia Barbon,
Martina Contran,
Damiana Incendi,
Filippo Perozzo,
Andrea Porzionato,
Vincenzo Vindigni,
Marco Patruno
Affiliations
Anna Carolo
Department of Comparative Biomedicine and Food Science, University of Padua, 35020 Legnaro, Italy
Luca Melotti
Department of Comparative Biomedicine and Food Science, University of Padua, 35020 Legnaro, Italy
Giulia Zivelonghi
Department of Comparative Biomedicine and Food Science, University of Padua, 35020 Legnaro, Italy
Roberta Sacchetto
Department of Comparative Biomedicine and Food Science, University of Padua, 35020 Legnaro, Italy
Eylem Emek Akyürek
Department of Comparative Biomedicine and Food Science, University of Padua, 35020 Legnaro, Italy
Tiziana Martinello
Department of Veterinary Medicine, University of Bari, 70010 Valenzano, Italy
Andrea Venerando
Department of Agricultural, Food, Environmental and Animal Sciences, University of Udine, 33100 Udine, Italy
Ilaria Iacopetti
Department of Animal Medicine, Production and Health, University of Padova, 35020 Legnaro, Italy
Michela Sugni
Department of Environmental Science and Policy, University of Milan, 20133 Milan, Italy
Giordana Martinelli
Department of Environmental Science and Policy, University of Milan, 20133 Milan, Italy
Margherita Roncoroni
Department of Environmental Science and Policy, University of Milan, 20133 Milan, Italy
Stefania Marzorati
Department of Environmental Science and Policy, University of Milan, 20133 Milan, Italy
Silvia Barbon
Department of Neuroscience, University of Padua, 35121 Padova, Italy
Martina Contran
Department of Neuroscience, University of Padua, 35121 Padova, Italy
Damiana Incendi
Department of Neuroscience, University of Padua, 35121 Padova, Italy
Filippo Perozzo
Plastic and Reconstructive Surgery Unit, Padova University Hospital, 35128 Padova, Italy
Andrea Porzionato
Department of Neuroscience, University of Padua, 35121 Padova, Italy
Vincenzo Vindigni
Department of Neuroscience, University of Padua, 35121 Padova, Italy
Marco Patruno
Department of Comparative Biomedicine and Food Science, University of Padua, 35020 Legnaro, Italy
The mutable collagenous tissue (MCT) of echinoderms possesses biological peculiarities that facilitate native collagen extraction and employment for biomedical applications such as regenerative purposes for the treatment of skin wounds. Strategies for skin regeneration have been developed and dermal substitutes have been used to cover the lesion to facilitate cell proliferation, although very little is known about the application of novel matrix obtained from marine collagen. From food waste we isolated eco-friendly collagen, naturally enriched with glycosaminoglycans, to produce an innovative marine-derived biomaterial assembled as a novel bi-layered skin substitute (Marine Collagen Dermal Template or MCDT). The present work carried out a preliminary experimental in vivo comparative analysis between the MCDT and Integra, one of the most widely used dermal templates for wound management, in a rat model of full-thickness skin wounds. Clinical, histological, and molecular evaluations showed that the MCDT might be a valuable tool in promoting and supporting skin wound healing: it is biocompatible, as no adverse reactions were observed, along with stimulating angiogenesis and the deposition of mature collagen. Therefore, the two dermal templates used in this study displayed similar biocompatibility and outcome with focus on full-thickness skin wounds, although a peculiar cellular behavior involving the angiogenesis process was observed for the MCDT.
