CELLULAR AND SUBCELLULAR LEVEL INVESTIGATION OF BIOLOGICAL OBJECTS BY MEANS OF FEMTOSECOND LASER OPTICAL TWEEZERS-SCALPEL

Abstract

The aim of this work was developing of elements of the precise three-dimensional positioning technology of one or several micron and submicron size biological objects. Thereto a laboratory unit of hardware-software complex of optical femtosecond laser tweezers-scalpel was developed and constructed in the Joint institute for high temperatures RAS using material resources of Russia. Experimental data concerning a maximal manipulation speed of CHO and cells, produced from mammalian spinal ganglia (using protocols for producing pure culture of Schwann cells) was received. Besides facts of interaction of laser radiation with intracellular structures that lead to unexpected behavior of cell in the zone of optical trap and change of maximal speed of cell manipulation were determined.

Keywords

• optical manipulation
• chinese hamster ovary cells
• schwann cells
• femtosecond laser
• tissue engineering