Isolation and identification of pathogenic bacteria from genital tract of the Arabian mares affected with genital tract infection and antimicrobial sensitivity

Abstract

This study was conducted for isolation and identification of the pathogenic bacteria presented in the genital tract infectionof the Arabian mares, and shows the anti microbial sensitivity. The study included 75 samples taken from infected maressuffering from genital tract infection diagnosed on the basis of case history and clinical signs which included bloody purulentdischarge ranched from yellow to green in colure, fetid oder with congested and oedematous vagina and from some abortioncases, and from mares suffered from tetanus disease symptoms during the period between October 2007 to April 2008 in studfarms breeding mares in Mosul. The samples were collected by swabs from the clitoris, clitorial fossa and the vagina. Isolationof bacteria was performed using aerobic and anaerobic culture techniques. Results of the present study showed a total ofisolation 75% from all samples taken with a high percentage isolation of Clostridium tetani (16.6%), followed by Archanobacterium pyogenes (10.6%), and Pseudomonas aeruginosa (8%), (6.7%) for each Enterobacter aerogenes, Klebsiellapneumonia, Streptococcus dysagalactiae subsp equisimilis, and (5.3%) for each bacteria Actinobacillus equilli, Streptococcuszooepidemicus, Staphylococcus aureus, then Proteus vulgaris (2.6%), and Escherichia coli (1.3%). The most bacterial isolateswere resistant to amoxicillin (100%), ampicillin (90.9 %), and erythromycin (65.9%), while the most isolates were sensitive tokanamycin (70.4%). It could be concluted that the most important bacteria causing genital tract infection of mares could beClostridium tetani and Archanobacterium pyogenes, Pseudomonas aeruginosa. The most bacterial isolates were resistant toamoxicillin, ampicillin and erythromycin.