Neurorestorative clinical application standards for the culture and quality control of neural progenitor/precursor cells (version 2017)

Abstract

Shiqing Feng,1,* Juan Xiao,2,3,* Fabin Han,4 Lin Chen,5 Wenyong Gao,2,3 Gengsheng Mao,2 Hongyun Huang2,3 On behalf of Neurorestoratology Professional Committee of Chinese Medical Doctors Association (Chinese Association of Neurorestoratology) 1International Science and Technology Cooperation Base of Spinal Cord Injury, Department of Orthopedics, Tianjin Medical University General Hospital, Tianjin 300052, People’s Republic of China; 2Institute of Neurorestoratology, The General Hospital of Chinese People’s Armed Police Forces, Beijing 100039, People’s Republic of China; 3Cell Therapy Center, Beijing Hongtianji Neuroscience Academy, Beijing 100043, People’s Republic of China; 4The Institute for Tissue Engineering and Regenerative Medicine, Liaocheng University/Liaocheng People’s Hospital, Liaocheng 252000, People’s Republic of China; 5Department of Neurosurgery, Tsinghua University Yuquan Hospital, Beijing 100040, People’s Republic of China *These authors contributed equally to this work Abstract: In order to promote the clinical use of neural progenitor or precursor cells for treating neurological diseases and damage, we need to standardize culture procedures for these cells. The Chinese Association of Neurorestoratology put forward these standards for training operators, standardized use and management of materials and equipment, standardized isolation and culture for neural progenitor/precursor cells, and the standardized management in preservation, transport, and related safe operation procedures of the neural progenitors. These cultures and quality control standards also include the Good Manufacturing Practice environment, routine maintenance as well as the monitoring and reporting of the clinical-grade neural progenitor cells. The aim of these standards is to improve the therapeutic efficacy and minimize the possible side effects from lake of quality control. Keywords: standardization, cell culture, quality control, neural progenitor cells, neural precursor cells, neurorestoratology

Keywords

• standardization
• cell culture
• quality control
• neural progenitor cells
• neural precursor cells
• neurorestoratology