Isolation and moleculer identification of thermophilic campylobacter species from mallard (anas platyrhynchos)

Abstract

Aim: The aim of this study was to investigate the Camplyobacter species which adversely affect human and animal health in mallard. Materials and Methods: 110 stool samples were collected from the mallard and examined by the cultural and molecular method. For pre-enrichment step, samples were inoculated with Preston Campylobacter Enrichment Broth. At the end of the incubation, preenriched culture was inoculated on Preston Campylobacter Selective Agar and the plates were incubated for 48-72 hours at 42°C. The cultures in which the growth was observed were first evaluated for the colony morphology and then for microscopic appearance. Suspected colonies were examined with multiplex PCR. Results: In this study, Campylobacter spp. was found in 10 (9.1%) of the 110 stool specimens. All 10 isolates were typed as Campylobacter jejuni by multiplex PCR. Conclusion: In this study, Campylobacter was detected in mallards and it was concluded that mallard may be an important reservoir for Campylobacter species. Besides, since Kars area is on the migration route of birds, it is important to examine the migratory birds and to determine the infections which birds have the potential.

Keywords

• thermophilic campylobacter
• isolation
• multiplex-pcr
• mallard