International Journal of Nanomedicine (Jan 2025)

Manipulated Slow Release of Florfenicol Hydrogels for Effective Treatment of Anti-Intestinal Bacterial Infections

  • Luo W,
  • Zhang M,
  • Jiang Y,
  • Ma G,
  • Liu J,
  • Dawood AS,
  • Xie S,
  • Algharib SA

Journal volume & issue
Vol. Volume 20
pp. 541 – 555

Abstract

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Wanhe Luo,1,2 Mengdi Zhang,1 Yongtao Jiang,1 Guocai Ma,3 Jinhuan Liu,1,4 Ali Sobhy Dawood,5 Shuyu Xie,6 Samah Attia Algharib7 1College of Animal Science and Technology, Tarim University, Alar, Xinjiang, 843300, People’s Republic of China; 2Key Laboratory of Livestock and Forage Resources Utilization Around Tarim, Ministry of Agriculture and Rural Areas, Tarim University, Alar, Xinjiang, 843300, People’s Republic of China; 3Instrumental Analysis Center, Tarim University, Alar, Xinjiang, 843300, People’s Republic of China; 4Lab for Sustainable Antimicrobials, Department of Pharmacy, Sichuan Agricultural University, Chengdu, Sichuan, 610000, People’s Republic of China; 5Medicine and Infectious Diseases Department, Faculty of Veterinary Medicine, University of Sadat City, Sadat city, 32897, Egypt; 6National Reference Laboratory of Veterinary Drug Residues (HZAU) and MARA Key Laboratory for Detection of Veterinary Drug Residues, Huazhong Agricultural University, Wuhan, Hubei, 430070, People’s Republic of China; 7Department of Clinical Pathology, Faculty of Veterinary Medicine, Benha University, Moshtohor, Toukh, 13736, EgyptCorrespondence: Wanhe Luo; Samah Attia Algharib, Email [email protected]; [email protected]: The difficulty of establishing slow release at intestinal infection sites, weak antibacterial effects, as well as the limited broad use of florfenicol oral formulations are the main targets of the current study. Novel hydrogels derived from sodium alginate were developed using a complexation form for florfenicol delivery to achieve slow release at the site of intestinal infection and enhance its antibacterial activity against Escherichia coli.Methods: The optimal formulation, physicochemical properties, stability, pH-responsive performance, antibacterial activity, and in vitro biosafety of the florfenicol hydrogels have been studied systematically.Results: The created hydrogels had a consistent spherical morphology, with an average diameter of 531.9± 12.6 nm. Energy dispersive spectroscopy and Fourier transform infrared indicated that florfenicol hydrogels have been successfully prepared through complexation force. Furthermore, it is shown that florfenicol hydrogels hold outstanding stability, excellent sustained release, and faster swelling and release at intestinal pH due to pH-responsiveness. The florfenicol hydrogels had no obvious structural destruction in simulated gastric juice (pH=1.2) for 12 hrs and were highly stable. However, the hydrogels began to be destroyed after 5 minutes in simulated intestinal fluid (SIF), and this decomposition was continuous. With the decomposition of the structure of florfenicol hydrogels, the encapsulated florfenicol was also slowly released, and thus, it achieves the slow-release effect. Additionally, the florfenicol hydrogels showed a low hemolytic ratio and greater antibacterial activity compared with florfenicol.Conclusion: The blended formulation creates a promising oral matrix intended for the slow-release of florfenicol along the gastrointestinal tract.Keywords: florfenicol, intestinal bacterial infections, hydrogels, sodium alginate, slow-release

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