The Effect of Toxigenic Strains of Helicobacter Pylori on the Severity of Chronic Gastroduodenal Pathology in Children

Abstract

The aim of the research was to study the incidence of virulent genotypes of Helicobacter pylori in children with chronic gastroduodenal pathology, as well as assessing their impact on severity of the disease. The study involved 280 children aged 8–17 years with chronic gastroduodenal pathology against the background of Helicobacter pylori infection. All patients underwent endoscopic examination with target biopsy of gastric and duodenal mucosa, and the subsequent morphological study of mucosal changes. The genotyping of Helicobacter pylori with detecting cagA, vacA, iceA and babA genes has been performed by polymerase chain reaction. The analysis of the genetic peculiarities of Helicobacter pylori showed a wide heterogeneity of microbe genome among examined children. It was found that virulent strains of Helicobacter pylori, which have cagA + vacAs1m1 or cagA + vacs1s2/m1m2 genotype, are specific for children with destructive changes in gastric and duodenal mucosa. In addition, the duration of chronic gastroduodenal pathology in children has a great impact on the severity of the inflammatory process in the mucous membrane. The presence of iceA and babA genes in the structure of Helicobacter pylori genome is not specific to children with chronic gastroduodenal pathology.

Keywords

• children
• Helicobacter pylori
• chronic gastroduodenal pathology
• virulent strains.