Nature Communications (Jan 2019)

An automated Bayesian pipeline for rapid analysis of single-molecule binding data

  • Carlas S. Smith,
  • Karina Jouravleva,
  • Maximiliaan Huisman,
  • Samson M. Jolly,
  • Phillip D. Zamore,
  • David Grunwald

DOI
https://doi.org/10.1038/s41467-018-08045-5
Journal volume & issue
Vol. 10, no. 1
pp. 1 – 9

Abstract

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Analysis of single-molecule binding assays still requires substantial manual user intervention. Here, the authors present a pipeline for rapid, automated analysis of co-localization single-molecule spectroscopy images, with a modular user interface that can be adjusted to a range of experimental conditions.