Cingulin and actin mediate midbody-dependent apical lumen formation during polarization of epithelial cells

Anthony J. Mangan; Daniel V. Sietsema; Dongying Li; Jeffrey K. Moore; Sandra Citi; Rytis Prekeris

doi:10.1038/ncomms12426

Nature Communications (Aug 2016)

Cingulin and actin mediate midbody-dependent apical lumen formation during polarization of epithelial cells

Anthony J. Mangan,
Daniel V. Sietsema,
Dongying Li,
Jeffrey K. Moore,
Sandra Citi,
Rytis Prekeris

Affiliations

Anthony J. Mangan: Department of Cell and Developmental Biology, School of Medicine, Anschutz Medical Campus, University of Colorado Denver
Daniel V. Sietsema: Department of Cell and Developmental Biology, School of Medicine, Anschutz Medical Campus, University of Colorado Denver
Dongying Li: Department of Cell and Developmental Biology, School of Medicine, Anschutz Medical Campus, University of Colorado Denver
Jeffrey K. Moore: Department of Cell and Developmental Biology, School of Medicine, Anschutz Medical Campus, University of Colorado Denver
Sandra Citi: Cell Biology Department, University of Geneva
Rytis Prekeris: Department of Cell and Developmental Biology, School of Medicine, Anschutz Medical Campus, University of Colorado Denver

DOI: https://doi.org/10.1038/ncomms12426
Journal volume & issue: Vol. 7, no. 1
pp. 1 – 15

Abstract

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Polarisation of epithelial cells causes lumen formation, which is mediated by apical membrane initiation site (AMIS) and FIP5, but how this is regulated is unclear. Here, the authors identify cingulin as a FIP-5 interacting protein, recruiting the Rac1-WAVE/Scar complex to the AMIS and branched actin formation.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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