Complete genome sequence of Lactobacillus rhamnosus Pen, a probiotic component of a medicine used in prevention of antibiotic-associated diarrhoea in children
Piotr Jarocki,
Marcin Podleśny,
Mariusz Krawczyk,
Agnieszka Glibowska,
Jarosław Pawelec,
Elwira Komoń-Janczara,
Oleksandr Kholiavskyi,
Michał Dworniczak,
Zdzisław Targoński
Affiliations
Piotr Jarocki
Department of Biotechnology, Microbiology and Human Nutrition, University of Life Sciences in Lublin
Marcin Podleśny
Department of Biotechnology, Microbiology and Human Nutrition, University of Life Sciences in Lublin
Mariusz Krawczyk
Genomed SA
Agnieszka Glibowska
Department of Biotechnology, Microbiology and Human Nutrition, University of Life Sciences in Lublin
Jarosław Pawelec
Laboratory of Electron Microscopy, Department of Comparative Anatomy and Anthropology, Maria Curie-Skłodowska University
Elwira Komoń-Janczara
Department of Biotechnology, Microbiology and Human Nutrition, University of Life Sciences in Lublin
Oleksandr Kholiavskyi
Department of Biotechnology, Microbiology and Human Nutrition, University of Life Sciences in Lublin
Michał Dworniczak
Department of Biotechnology, Microbiology and Human Nutrition, University of Life Sciences in Lublin
Zdzisław Targoński
Department of Biotechnology, Microbiology and Human Nutrition, University of Life Sciences in Lublin
Abstract Background Lactobacillus rhamnosus Pen is a human endogenous strain with well-documented health promoting properties that is used for production of probiotics. It has a long safety history of application, and its effectiveness in the prevention of antibiotic-associated diarrhoea has also been confirmed in clinical trials. Results Here we present the complete genome sequence of L. rhamnosus Pen, which consists of a circular 2,884,4966-bp chromosome with a GC content of 46.8%. Within 2907 open reading frames (ORFs), genes involved with probiotic properties were identified. A CRISPR locus, consisting of a 1092-nt region with 16 spacers, was also detected. Finally, an intact prophage of ~ 40.7 kb, 57 ORFs, GC content 44.8% was identified. Conclusions Genomic analysis confirmed the probiotic properties of L. rhamnosus Pen and may indicate new biotechnological applications of this industrially important strain.
