CHD4 slides nucleosomes by decoupling entry- and exit-side DNA translocation

Yichen Zhong; Bishnu P. Paudel; Daniel P. Ryan; Jason K. K. Low; Charlotte Franck; Karishma Patel; Max J. Bedward; Mario Torrado; Richard J. Payne; Antoine M. van Oijen; Joel P. Mackay

doi:10.1038/s41467-020-15183-2

Nature Communications (Mar 2020)

CHD4 slides nucleosomes by decoupling entry- and exit-side DNA translocation

Yichen Zhong,
Bishnu P. Paudel,
Daniel P. Ryan,
Jason K. K. Low,
Charlotte Franck,
Karishma Patel,
Max J. Bedward,
Mario Torrado,
Richard J. Payne,
Antoine M. van Oijen,
Joel P. Mackay

Affiliations

Yichen Zhong: School of Life and Environmental Sciences, University of Sydney
Bishnu P. Paudel: Molecular Horizons, School of Chemistry and Molecular Bioscience, University of Wollongong
Daniel P. Ryan: Department of Genome Sciences, The John Curtin School of Medical Research, The Australian National University
Jason K. K. Low: School of Life and Environmental Sciences, University of Sydney
Charlotte Franck: School of Life and Environmental Sciences, University of Sydney
Karishma Patel: School of Life and Environmental Sciences, University of Sydney
Max J. Bedward: School of Life and Environmental Sciences, University of Sydney
Mario Torrado: School of Life and Environmental Sciences, University of Sydney
Richard J. Payne: School of Chemistry, The University of Sydney
Antoine M. van Oijen: Molecular Horizons, School of Chemistry and Molecular Bioscience, University of Wollongong
Joel P. Mackay: School of Life and Environmental Sciences, University of Sydney

DOI: https://doi.org/10.1038/s41467-020-15183-2
Journal volume & issue: Vol. 11, no. 1
pp. 1 – 14

Abstract

Read online

Chromatin remodellers hydrolyse ATP to move nucleosomal DNA against histone octamers. Here, the authors use single-molecule assays to examine the mechanism of action of CHD4 remodeller, and provide evidence that CHD4 slides nucleosomes by decoupling entry- and exit-side DNA translocation.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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