Interactions between stimuli-evoked cortical activity and spontaneous low frequency oscillations measured with neuronal calcium

Abstract

Spontaneous brain activity has been widely used to map brain connectivity. The interactions between task-evoked brain responses and the spontaneous cortical oscillations, especially within the low frequency range of ~0.1 ​Hz, are not fully understood. Trial-to-trial variabilities in brain’s response to sensory stimuli and the ability for brain to detect under noisy conditions suggest an appreciable impact of the brain state. Using a multimodality imaging platform, we simultaneously imaged neuronal Ca2+ and cerebral hemodynamics at baseline and in response to single-pulse forepaw stimuli in rat’s somatosensory cortex. The high sensitivity of this system enables detection of responses to very weak and strong stimuli and real time determination of low frequency oscillations without averaging. Results show that the ongoing neuronal oscillations inversely modulate Ca2+ transients evoked by sensory stimuli. High intensity stimuli reset the spontaneous neuronal oscillations to an unpreferable excitability following the stimulus. Cerebral hemodynamic responses also inversely interact with the spontaneous hemodynamic oscillations, correlating with the neuronal Ca2+ transient changes. The results reveal competing interactions between spontaneous oscillations and stimulation-evoked brain activities in somatosensory cortex and the resultant hemodynamics.