Taeanamides A and B, Nonribosomal Lipo-Decapeptides Isolated from an Intertidal-Mudflat-Derived <i>Streptomyces</i> sp.
Jinsheng Cui,
Eunji Kim,
Dong Hyun Moon,
Tae Ho Kim,
Ilnam Kang,
Yeonjung Lim,
Daniel Shin,
Sunghoon Hwang,
Young Eun Du,
Myoung Chong Song,
Munhyung Bae,
Jang-Cheon Cho,
Jichan Jang,
Sang Kook Lee,
Yeo Joon Yoon,
Dong-Chan Oh
Affiliations
Jinsheng Cui
Natural Products Research Institute, College of Pharmacy, Seoul National University, Seoul 08826, Korea
Eunji Kim
Natural Products Research Institute, College of Pharmacy, Seoul National University, Seoul 08826, Korea
Dong Hyun Moon
Natural Products Research Institute, College of Pharmacy, Seoul National University, Seoul 08826, Korea
Tae Ho Kim
Molecular Mechanism of Antibiotics, Division of Life Science, Department of Bio & Medical Big Data (BK4 Program), Research Institute of Life Science, Gyeongsang National University, Jinju 52828, Korea
Ilnam Kang
Department of Biological Sciences, Inha University, Incheon 22212, Korea
Yeonjung Lim
Department of Biological Sciences, Inha University, Incheon 22212, Korea
Daniel Shin
Natural Products Research Institute, College of Pharmacy, Seoul National University, Seoul 08826, Korea
Sunghoon Hwang
Natural Products Research Institute, College of Pharmacy, Seoul National University, Seoul 08826, Korea
Young Eun Du
Natural Products Research Institute, College of Pharmacy, Seoul National University, Seoul 08826, Korea
Myoung Chong Song
Natural Products Research Institute, College of Pharmacy, Seoul National University, Seoul 08826, Korea
Munhyung Bae
College of Pharmacy, Gachon University, Incheon 21936, Korea
Jang-Cheon Cho
Department of Biological Sciences, Inha University, Incheon 22212, Korea
Jichan Jang
Molecular Mechanism of Antibiotics, Division of Life Science, Department of Bio & Medical Big Data (BK4 Program), Research Institute of Life Science, Gyeongsang National University, Jinju 52828, Korea
Sang Kook Lee
Natural Products Research Institute, College of Pharmacy, Seoul National University, Seoul 08826, Korea
Yeo Joon Yoon
Natural Products Research Institute, College of Pharmacy, Seoul National University, Seoul 08826, Korea
Dong-Chan Oh
Natural Products Research Institute, College of Pharmacy, Seoul National University, Seoul 08826, Korea
Two new lipo-decapeptides, namely taeanamides A and B (1 and 2), were discovered from the Gram-positive bacterium Streptomyces sp. AMD43, which was isolated from a mudflat sample from Anmyeondo, Korea. The exact molecular masses of 1 and 2 were revealed by high-resolution mass spectrometry, and the planar structures of 1 and 2 were elucidated using NMR spectroscopy. The absolute configurations of 1 and 2 were determined using a combined analysis of 1H-1H coupling constants and ROESY correlations, the advanced Marfey’s method, and bioinformatics. The putative nonribosomal peptide synthetase pathway for the taeanamides was identified by analyzing the full genome sequence data of Streptomyces sp. AMD43. We also found that taeanamide A exhibited mild anti-tuberculosis bioactivity, whereas taeanamide B showed significant bioactivity against several cancer cell lines.
