Multifunctional dietary approach reduces intestinal inflammation in relation with changes in gut microbiota composition in subjects at cardiometabolic risk: the SINFONI project
Hugo Hornero-Ramirez,
Arianne Morissette,
Bruno Marcotte,
Armelle Penhoat,
Béryle Lecomte,
Baptiste Panthu,
Jacob Lessard Lord,
Florence Thirion,
Laurie Van-Den-Berghe,
Emilie Blond,
Chantal Simon,
Cyrielle Caussy,
Nathalie Feugier,
Joël Doré,
Philippe Sanoner,
Alexandra Meynier,
Yves Desjardins,
Geneviève Pilon,
André Marette,
Patrice D. Cani,
Martine Laville,
Sophie Vinoy,
Marie-Caroline Michalski,
Julie-Anne Nazare
Affiliations
Hugo Hornero-Ramirez
Centre de Recherche en Nutrition Humaine - Rhône-Alpes, INSERM, INRAe, Université Claude Bernard Lyon1, Hospices Civils de Lyon, Pierre Bénite, France
Arianne Morissette
Institute of Nutrition and Functional Foods (INAF), Faculty of Agriculture and Food Sciences, Laval University, Québec, QC, Canada
Bruno Marcotte
Institute of Nutrition and Functional Foods (INAF), Faculty of Agriculture and Food Sciences, Laval University, Québec, QC, Canada
Armelle Penhoat
Laboratoire CarMeN, INSERM U.1060, INRAe U. 1397, Université Claude Bernard Lyon1, Pierre Bénite, France
Béryle Lecomte
Laboratoire CarMeN, INSERM U.1060, INRAe U. 1397, Université Claude Bernard Lyon1, Pierre Bénite, France
Baptiste Panthu
Laboratoire CarMeN, INSERM U.1060, INRAe U. 1397, Université Claude Bernard Lyon1, Pierre Bénite, France
Jacob Lessard Lord
INRAE, MGP, Université Paris-Saclay, Jouy-en-Josas, France
Florence Thirion
INRAE, MGP, Université Paris-Saclay, Jouy-en-Josas, France
Laurie Van-Den-Berghe
Centre de Recherche en Nutrition Humaine - Rhône-Alpes, INSERM, INRAe, Université Claude Bernard Lyon1, Hospices Civils de Lyon, Pierre Bénite, France
Emilie Blond
Laboratoire CarMeN, INSERM U.1060, INRAe U. 1397, Université Claude Bernard Lyon1, Pierre Bénite, France
Chantal Simon
Centre de Recherche en Nutrition Humaine - Rhône-Alpes, INSERM, INRAe, Université Claude Bernard Lyon1, Hospices Civils de Lyon, Pierre Bénite, France
Cyrielle Caussy
Centre de Recherche en Nutrition Humaine - Rhône-Alpes, INSERM, INRAe, Université Claude Bernard Lyon1, Hospices Civils de Lyon, Pierre Bénite, France
Nathalie Feugier
Centre de Recherche en Nutrition Humaine - Rhône-Alpes, INSERM, INRAe, Université Claude Bernard Lyon1, Hospices Civils de Lyon, Pierre Bénite, France
Joël Doré
INRAE, MGP, Université Paris-Saclay, Jouy-en-Josas, France
Philippe Sanoner
iSymrise-Diana Food SAS, R&D, Naturals Food & Beverage, Rennes, France
Alexandra Meynier
Nutrition Research, Paris-Saclay Tech Center, Mondelez International R&D, Saclay, France
Yves Desjardins
Institute of Nutrition and Functional Foods (INAF), Faculty of Agriculture and Food Sciences, Laval University, Québec, QC, Canada
Geneviève Pilon
Department of Medicine, Faculty of Medicine, Québec Heart and Lung Institute, Université Laval, Québec, Canada
André Marette
Department of Medicine, Faculty of Medicine, Québec Heart and Lung Institute, Université Laval, Québec, Canada
Patrice D. Cani
UCLouvain, Université catholique de Louvain, Louvain Drug Research Institute, (LDRI) Metabolism and Nutrition Research Group (MNUT), Brussels, Belgium
Martine Laville
Centre de Recherche en Nutrition Humaine - Rhône-Alpes, INSERM, INRAe, Université Claude Bernard Lyon1, Hospices Civils de Lyon, Pierre Bénite, France
Sophie Vinoy
Nutrition Research, Paris-Saclay Tech Center, Mondelez International R&D, Saclay, France
Marie-Caroline Michalski
Laboratoire CarMeN, INSERM U.1060, INRAe U. 1397, Université Claude Bernard Lyon1, Pierre Bénite, France
Julie-Anne Nazare
Centre de Recherche en Nutrition Humaine - Rhône-Alpes, INSERM, INRAe, Université Claude Bernard Lyon1, Hospices Civils de Lyon, Pierre Bénite, France
The development of cardiometabolic (CM) diseases is associated with chronic low-grade inflammation, partly linked to alterations of the gut microbiota (GM) and reduced intestinal integrity. The SINFONI project investigates a multifunctional (MF) nutritional strategy’s impact combining different bioactive compounds on inflammation, GM modulation and CM profile. In this randomized crossover-controlled study, 30 subjects at CM-risk consumed MF cereal-products, enriched with polyphenols, fibers, slowly-digestible starch, omega-3 fatty acids or Control cereal-products (without bioactive compounds) for 2 months. Metabolic endotoxemia (lipopolysaccharide (LPS), lipopolysaccharide-binding protein over soluble cluster of differentiation-14 (LBP/sCD14), systemic inflammation and cardiovascular risk markers, intestinal inflammation, CM profile and response to a one-week fructose supplementation, were assessed at fasting and post mixed-meal. GM composition and metabolomic analysis were conducted. Mixed linear models were employed, integrating time (pre/post), treatment (MF/control), and sequence/period. Compared to control, MF intervention reduced intestinal inflammation (fecal calprotectin, p = 0.007) and endotoxemia (fasting LPS, p < 0.05), without alteration of systemic inflammation. MF decreased serum branched-chain amino acids compared to control (p < 0.05) and increased B.ovatus, B.uniformis, A.butyriciproducens and unclassified Christensenellaceae.CAG-74 (p < 0.05). CM markers were unchanged. A 2-month dietary intervention combining multiple bioactive compounds improved intestinal inflammation and induced GM modulation. Such strategy appears as an effective strategy to target low-grade inflammation through multi-target approach.
