Treatment of gastric cancer by silver nanoparticles encapsulated by chitosan polymers mediated by Pistacia atlantica extract under ultrasound condition

Abstract

In this study, we prepared silver nanoparticles (AgNPs) employing ultrasound and chitosan (CS) in the presence of Pistacia atlantica aqueous extract as a non-toxic stabilizing and reducing agent for capping AgNPs with CS in a simple single-step process. The reduction of Ag+ ions into Ag0NPs was indicated by a visual transform in color. UV-Vis studies revealed that the characteristic surface plasmon resonance band appeared at around 460 nm. FT-IR, TEM, SEM, EDX, elemental mapping, XRD, and UV/Vis analytical methods were employed to characterize the AgNPs@CS nanocomposite. TEM study revealed nanoflowers-shaped NPs having various particle sizes. In the oncological part of this research, the status of normal and gastric cancer cell lines (MKN45, MKN28, NCI-N87, SNU-16, SNU-5, SNU-1, AGS, and KATO III) against the proposed nanomaterial were determined. The IC50 of AgNPs was 265, 304, 325, 127, 297, 318, 125, and 114 µg/mL against MKN45, MKN28, NCI-N87, SNU-16, SNU-5, SNU-1, AGS, and KATO III, respectively. It seems that the prepared NPs have stopped the growth of gastric cancer cells and the recent cancer cells have been removed with a high concentration of NPs. DPPH test results indicated removing 50% radicals by nanocomposite and butylated hydroxytoluene in the doses of 183 and 45 μg/mL.

Keywords

• chitosan
• pistacia atlantica
• silver
• agnps@cs
• anti-gastric cancer
• cytotoxicity